Overview

  • Product name

  • Description

    Goat polyclonal to VGluT1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide HDQLAGSDDSEMED(Human)

  • Positive control

    • Human brain (cortex) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab110139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity

    Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGLUT 1 antibody
    • VGluT1 antibody
    see all

Images

  • ab110139, at 3.75 µg/ml, staining VGluT1 in Human brain (cortex) tissue by immunohistochemistry. After incubation with ab110139, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab110139 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (synaptosome particles made from human brain)
Permeabilization
Yes - tween
Gating Strategy
Synaptosomes gated based on size
Specification
synaptosome particles made from human brain
Preparation
Cell harvesting/tissue preparation method: tissue homogenized and centrifuged to isolate synaptic particles
Sample buffer: PBS
Fixation
Paraformaldehyde

Emily Sherfield

Verified customer

Submitted Jun 28 2016

Answer

I am sorry these are the only products we can recommend. The reason for recommendation is that there is very low sequence homology between the N-terminal and C-terminal end of vglut1 and vglut2. So I am presuming that antibodies against these sequences will detect the distinct proteins. ab110139 has not been tested in mouse species however if you check the immunogen sequence homology between mouse and human then it is 100% so it is more likely that this antibody will cross react and detect the mouse protein also. Mouse is an untested species so again this antibody is also eligible for Abreview discount programme. The validity of discount code is 4 months. So whenever you plan for experiment let me know I will create a discount code for you. Please be also advised that in the mean time if we receive tested data for these species or application then these products will no longer be eligible for discount code. I hope this will be helpful. Should you have any other question please do not hesitate to contact me. PS: Please check Swissprot and NCBI database for sequence homology.

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Answer

It is true that ab84103 wasn't tested in ICC/IF however it does not mean, it can't be used. It only means we do not have tested data to confirm it. If you however you would like to use ab84103 in ICC/IF then I can offer you a Abreview testing discount; with this discount you can actually get a primary antibody free of charge in exchange of ICC/IF Abreview. The free of charge antibody will be of your next purchase however you have to purchase the current antibody at full price. Let me know if you are interested in this offer then I will be happy in sending the discount code. I hope this will help. Should you have any other please do not hesitate to contact me.

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Answer

Thank you for contacting us. The epitope of ab79157 has not been mapped so we do not know the degree of homology shared by epitope in vGlut1 and vGlut2 proteins. The predicted band size for vGlu2 protein is 64 kDa. I do agree that there are faint bands at 75 kDa and 50 kDa. I believe that with protocol optimizations the results could have been improved. We have used 10 ug of lysates while testing this antibody however we always recommend our customers using 30-40 ug of lysates per lane. We have used this antibody at 1/1000 dilution so we also recommend trying this antibody at range of dilutions e.g. 1/100, 1/500 and 1/1000 for optimized results. Ab79157 is fully guaranteed for detection of vGlut2; if however this product fails in applications as stated on the datasheet we will be happy to refund the money you pay. We have 8 Abreviews for ab72311 which shows it is a good product. The c terminal end of vGlut1 and vGLut2 does not show homology so this product could also be good in detection on only vGlut1. Please be advised that our answer is only based on sequence homology data; we do not have cross reactivity tested data that we can share. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. The N-terminal and C-terminal region amino acids of these proteins do not show sequence homology so the antibodies against the N or C terminal sequence will detect distinct protein they are raised against. Ab110139 and ab84103 immunogen show very low homology between each other. So these could be the products you are looking for. I am sorry we have not done any in-house cross reactivity tests with these products. Our assumption is based only on immunogen sequence homology. We will not be able to provide any tested data regarding this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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