• Product name
  • Description
    Rabbit polyclonal to VGluT1
  • Host species
  • Specificity
    Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%).
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Zebrafish
    Predicted to work with: Mouse, Cow, Pigeon, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Rat VGluT1.

    (Peptide available as ab93078.)

  • Positive control
    • This antibody gave a positive signal in the following rat tissue lysates: Brain; Cerrebellum; Cortex; Forebrain.



Our Abpromise guarantee covers the use of ab77822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).


  • Function
    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity
    Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGluT1 antibody
    see all


  • IHC-Fr image of VGluT1 staining on zebrafish retina sections using ab77822 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab77822 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml

    Lane 1 : Rat Brain Tissue Lysate
    Lane 2 : Rat Cerebellum Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Rat Forebrain

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 67 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes

    Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.


This product has been referenced in:
  • D'Abaco GM  et al. Graphene foam as a biocompatible scaffold for culturing human neurons. R Soc Open Sci 5:171364 (2018). Read more (PubMed: 29657752) »
  • Wang HS  et al. Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury. Neurochem Int 99:9-15 (2016). WB . Read more (PubMed: 27210824) »

See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Zebrafish Tissue sections (Brain, axons)
Brain, axons
Yes - Triton X

Abcam user community

Verified customer

Submitted Aug 26 2014

DISCOUNT CODES: ab5981 - ******
ab104898 - ***********

Expiration date: 9th July 2012 (for both discount codes)

I am very pleased to hear you would like to accept our offer and test ab5981 and ab104898in hamster tissue]. This ...

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Thank you for your inquiry. The above mentioned antibodies are all tested to be specific for either VGlut1 or VGlut2. The sequence homology is only 73% between the two VGlut proteins and the antibodies are all tested to be specific for either VGlut...

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