Anti-VGluT1 antibody (ab77822)
Key features and details
- Rabbit polyclonal to VGluT1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VGluT1 antibody
See all VGluT1 primary antibodies -
Description
Rabbit polyclonal to VGluT1 -
Host species
Rabbit -
Specificity
Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Pigeon, Human, Zebrafish -
Immunogen
Synthetic peptide corresponding to Rat VGluT1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93078) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
Tissue specificity
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. -
Sequence similarities
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 518849 Cow
- Entrez Gene: 57030 Human
- Entrez Gene: 72961 Mouse
- Entrez Gene: 116638 Rat
- Omim: 605208 Human
- SwissProt: A4FV52 Cow
- SwissProt: Q9P2U7 Human
- SwissProt: Q3TXX4 Mouse
see all -
Alternative names
- BNPI antibody
- Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
- Brain specific Na dependent inorganic phosphate cotransporter antibody
see all
Images
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All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Cerebellum Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab77822 has been referenced in 21 publications.
- Albrecht NE et al. Rapid 3D-STORM imaging of diverse molecular targets in tissue. Cell Rep Methods 2:100253 (2022). PubMed: 35880013
- Garita-Hernandez M et al. Control of Microbial Opsin Expression in Stem Cell Derived Cones for Improved Outcomes in Cell Therapy. Front Cell Neurosci 15:648210 (2021). PubMed: 33815066
- DeMarco E et al. Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis. Front Neuroanat 15:636683 (2021). PubMed: 33613200
- Shallie PD et al. Early glutathione intervention educed positive correlation between VGLUT1 expression and spatial memory in the N?-nitro-L-arginine methyl rat model of IUGR. IBRO Neurosci Rep 10:136-141 (2021). PubMed: 34179867
- Willsey HR et al. Parallel in vivo analysis of large-effect autism genes implicates cortical neurogenesis and estrogen in risk and resilience. Neuron 109:788-804.e8 (2021). PubMed: 33497602