Overview

  • Product name

    Anti-VGluT1 antibody [N28-9]
    See all VGluT1 primary antibodies
  • Description

    Mouse monoclonal [N28-9] to VGluT1
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against VGluT2.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat VGluT1 aa 450-600 (C terminal).

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Brain tissue lysates in Western Blot, and on a formalin-fixed, paraffin-embedded Rat Brain Tissue section in IHC.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab134283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity

    Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGluT1 antibody
    see all

Images

  • All lanes : Anti-VGluT1 antibody [N28-9] (ab134283) at 5 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 50,55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134283 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • All lanes : Anti-VGluT1 antibody [N28-9] (ab134283) at 5 µg

    Lane 1 : Human Brain Tissue Lysate
    Lane 2 : Rat Brain Tissue Lysate
    Lane 3 : Mouse Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 50,55 kDa why is the actual band size different from the predicted?
    Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134283 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of VGluT1 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Torres L  et al. Toxoplasma gondii alters NMDAR signaling and induces signs of Alzheimer's disease in wild-type, C57BL/6 mice. J Neuroinflammation 15:57 (2018). Read more (PubMed: 29471842) »
  • Zaric M  et al. Regional-specific effects of cerebral ischemia/reperfusion and dehydroepiandrosterone on synaptic NMDAR/PSD-95 complex in male Wistar rats. Brain Res 1688:73-80 (2018). Read more (PubMed: 29577884) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 04 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Retina, plexiform layers)
Permeabilization
Yes - Triton X
Specification
Retina, plexiform layers
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 05 2014

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