Key features and details
- Rabbit polyclonal to VGLUT2 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-VGLUT2 antibody - C-terminal
See all VGLUT2 primary antibodies
DescriptionRabbit polyclonal to VGLUT2 - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within Mouse VGLUT2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q8BLE7
- WB: Mouse and rat brain extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
FunctionMediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Tissue specificityPredominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
Sequence similaritiesBelongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
Developmental stageExpressed in fetal brain.
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
- Information by UniProt
- Differentiation associated BNPI antibody
- Differentiation associated Na dependent inorganic phosphate cotransporter antibody
- Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
All lanes : Anti-VGLUT2 antibody - C-terminal (ab229711) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for VGLUT2 (green) using ab229711 at 1/500 dilution in ICC/IF.
Nuclear counterstain: DAPI (blue).
beta Tubulin 3, stained by anti-beta Tubulin 3 antibody at 1/500 dilution (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229711 has not yet been referenced specifically in any publications.