Product nameAnti-VGLUT2 antibody - C-terminal
See all VGLUT2 primary antibodies
DescriptionRabbit polyclonal to VGLUT2 - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within Mouse VGLUT2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q8BLE7
- WB: Mouse and rat brain extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
FunctionMediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Tissue specificityPredominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
Sequence similaritiesBelongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
Developmental stageExpressed in fetal brain.
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
- Information by UniProt
- Differentiation associated BNPI antibody
- Differentiation associated Na dependent inorganic phosphate cotransporter antibody
- Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
All lanes : Anti-VGLUT2 antibody - C-terminal (ab229711) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for VGLUT2 (green) using ab229711 at 1/500 dilution in ICC/IF.
Nuclear counterstain: DAPI (blue).
beta Tubulin 3, stained by anti-beta Tubulin 3 antibody at 1/500 dilution (red).
ab229711 has not yet been referenced specifically in any publications.