Overview

  • Product name

    Anti-VGLUT2 antibody - C-terminal
    See all VGLUT2 primary antibodies
  • Description

    Rabbit polyclonal to VGLUT2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse VGLUT2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q8BLE7

  • Positive control

    • WB: Mouse and rat brain extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity

    Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Differentiation associated BNPI antibody
    • Differentiation associated Na dependent inorganic phosphate cotransporter antibody
    • Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
    • Differentiation-associated BNPI antibody
    • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
    • DNPI antibody
    • SLC17A6 antibody
    • Sodium dependent inorganic phosphate cotransporter antibody
    • Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
    • Solute carrier family 17 member 6 antibody
    • Vesicular glutamate transporter 2 antibody
    • Vesicular glutamate transporter type 2 antibody
    • VGLU2_HUMAN antibody
    • VGLUT 2 antibody
    • VGluT2 antibody
    see all

Images

  • All lanes : Anti-VGLUT2 antibody - C-terminal (ab229711) at 1/1000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 65 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for VGLUT2 (green) using ab229711 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: DAPI (blue). 

    beta Tubulin 3, stained by anti-beta Tubulin 3 antibody at 1/500 dilution (red).

References

ab229711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229711.
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