Overview

  • Product name

    Anti-VGLUT2 antibody [EPR21085]
    See all VGLUT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21085] to VGLUT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse VGLUT2 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8BLE7

  • Positive control

    • WB: Mouse cerebral cortex, hypothalamus, hippocampus and brain lysates; Rat brain, cortex and cerebellum lysates. IHC-P: Mouse brain and cerebrum tissues; Rat brain tissue. IHC-Fr: Mouse thalamus tissue; Rat cortex tissue. IP: Mouse brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21085
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Use at 1/500 dilution for mouse and at 1/100 dilution for rat.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IP 1/30.

Target

  • Function

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity

    Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Differentiation associated BNPI antibody
    • Differentiation associated Na dependent inorganic phosphate cotransporter antibody
    • Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
    • Differentiation-associated BNPI antibody
    • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
    • DNPI antibody
    • SLC17A6 antibody
    • Sodium dependent inorganic phosphate cotransporter antibody
    • Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
    • Solute carrier family 17 member 6 antibody
    • Vesicular glutamate transporter 2 antibody
    • Vesicular glutamate transporter type 2 antibody
    • VGLU2_HUMAN antibody
    • VGLUT 2 antibody
    • VGluT2 antibody
    see all

Images

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of mouse thalamus tissue labeling VGLUT2 with ab216463  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in mouse thalamus.

    The nuclear counter stain is DAPI (blue). 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-VGLUT2 antibody [EPR21085] (ab216463) at 1/1000 dilution

    Lane 1 : Mouse cerebral cortex lysate
    Lane 2 : Mouse hypothalamus lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 24 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Target is expressed in neurons, with spleen acting as a negative control (PMID: 15682395, PMID: 15102489). Note that the lysates were not boiled prior to gel loading to avoid aggregation of this membrane protein. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • All lanes : Anti-VGLUT2 antibody [EPR21085] (ab216463) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat cortex lysate
    Lane 3 : Rat cerebellum lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Note that the lysates were not boiled prior to gel loading to avoid aggregation of this membrane protein. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse forebrain, including cerebral cortex and diencephalon is observed (PMID: 16217795). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granularly cytoplasmic staining in mouse cerebrum is observed (PMID: 24804702). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat forebrain including cerebral cortex and diencephalon is observed (PMID: 16217795). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of rat cortex tissue labeling VGLUT2 with ab216463  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in rat cortex.

    The nuclear counter stain is DAPI (blue). 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • VGLUT2 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab216463 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216463 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab216463 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216463 in mouse brain lysate.

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Lizarbe B  et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). Read more (PubMed: 30670942) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Adult Brain section (Striatum))
Antigen retrieval step
None
Permeabilization
Yes - Triton-X100 0.1%
Specification
Adult Brain section (Striatum)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

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Submitted Jan 26 2019

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