Recombinant
RabMAb

Recombinant Anti-VGLUT2 antibody [EPR21085] - BSA and Azide free (ab227906)

Overview

  • Product name

    Anti-VGLUT2 antibody [EPR21085] - BSA and Azide free
    See all VGLUT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21085] to VGLUT2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse VGLUT2 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8BLE7

  • Positive control

    • IHC-P: Mouse brain tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab227906 is a PBS-only buffer format of ab216463. Please refer to ab216463 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21085
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity

    Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Differentiation associated BNPI antibody
    • Differentiation associated Na dependent inorganic phosphate cotransporter antibody
    • Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
    • Differentiation-associated BNPI antibody
    • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
    • DNPI antibody
    • SLC17A6 antibody
    • Sodium dependent inorganic phosphate cotransporter antibody
    • Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
    • Solute carrier family 17 member 6 antibody
    • Vesicular glutamate transporter 2 antibody
    • Vesicular glutamate transporter type 2 antibody
    • VGLU2_HUMAN antibody
    • VGLUT 2 antibody
    • VGluT2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granularly cytoplasmic staining in mouse cerebrum is observed (PMID: 24804702). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat forebrain including cerebral cortex and diencephalon is observed (PMID: 16217795). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of mouse thalamus tissue labeling VGLUT2 with ab216463  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in mouse thalamus.

    The nuclear counter stain is DAPI (blue). 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of rat cortex tissue labeling VGLUT2 with ab216463  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in rat cortex.

    The nuclear counter stain is DAPI (blue). 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

  • VGLUT2 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab216463 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216463 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab216463 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216463 in mouse brain lysate.

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse forebrain, including cerebral cortex and diencephalon (PMID: 16217795). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216463).

References

ab227906 has not yet been referenced specifically in any publications.

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