Key features and details
- Mouse monoclonal [N34/34] to VGLUT3
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-VGLUT3 antibody [N34/34]
See all VGLUT3 primary antibodies
DescriptionMouse monoclonal [N34/34] to VGLUT3
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- ICC/IF: SK-N-BE cells.
The clone number has been updated from S34-34 to N34/34, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab134310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 65 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionMediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Tissue specificityExpressed in amygdala, cerebellum, hippocampus, medulla, spinal cord and thalamus.
Involvement in diseaseDefects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25) [MIM:605583]. DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age-dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged.
Sequence similaritiesBelongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Membrane. Cell junction > synapse > synaptosome.
- Information by UniProt
- deafness autosomal dominant 25 antibody
- DFNA 25 antibody
- DFNA25 antibody
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling VGLUT3 using ab134310 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
ab134310 staining VGLUT3 in human tendon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with 10% formalin and blocked for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134310 has not yet been referenced specifically in any publications.