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  1. Link

    vilip1-antibody-epr9833-ab151741.pdf

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Signal Transduction Second Messenger Nucleotide Messenger cAMP
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RecombinantRabMAb

Recombinant Anti-VILIP1 antibody [EPR9833] (ab151741)

  • Datasheet
Submit a review Q&A (1)References (1)

Product price, shipping and contact information

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Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
  • Anti-VILIP1 antibody [EPR9833] (ab151741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9833] to VILIP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human VILIP1 protein (ab63276)

View more associated products

Overview

  • Product name

    Anti-VILIP1 antibody [EPR9833]
    See all VILIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9833] to VILIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human VILIP1 (P62760).

  • Positive control

    • Human fetal brain and Human cerebellum lysates; Human brain and glioma tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9833
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (ab188106)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human VILIP1 protein (ab63276)

Applications

Our Abpromise guarantee covers the use of ab151741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
    • Tissue specificity

      Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level).
    • Sequence similarities

      Belongs to the recoverin family.
      Contains 4 EF-hand domains.
    • Target information above from: UniProt accession P62760 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7447 Human
      • Entrez Gene: 26950 Mouse
      • Entrez Gene: 24877 Rat
      • Omim: 600817 Human
      • SwissProt: P62760 Human
      • SwissProt: P62761 Mouse
      • SwissProt: P62762 Rat
      • Unigene: 444212 Human
      • Unigene: 27005 Mouse
      • Unigene: 105934 Rat
      see all
    • Alternative names

      • 21 kDa CABP antibody
      • Hippocalcin like protein 3 antibody
      • Hippocalcin-like protein 3 antibody
      • HLP 3 antibody
      • HLP3 antibody
      • HPCAL 3 antibody
      • HPCAL3 antibody
      • HUVISL1 antibody
      • Neural visinin-like protein 1 antibody
      • Neural visinin-like type 1 protein antibody
      • Neurocalcin alpha antibody
      • NVL 1 antibody
      • NVL1 antibody
      • Nvp1 antibody
      • OZ1 antibody
      • Ratnvp1 antibody
      • VILIP antibody
      • VILIP-1 antibody
      • Visinin antibody
      • Visinin like 1 antibody
      • Visinin like protein 1 antibody
      • Visinin-like protein 1 antibody
      • VISL 1 antibody
      • VISL1 antibody
      • VISL1_HUMAN antibody
      • VLP-1 antibody
      • Vnsl1 antibody
      • Vsnl1 antibody
      see all

    Images

    • Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)
      All lanes : Anti-VILIP1 antibody [EPR9833] (ab151741) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab151741 at 1/100 diluton.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling VILIP1 with ab151741 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin embedded Human Retina tissue using ab151741 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151741 showing -ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab151741 showing -ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

      Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151741 showing -ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-VILIP1 antibody [EPR9833] (ab151741)
      Anti-VILIP1 antibody [EPR9833] (ab151741)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab151741? Please let us know so that we can cite the reference in this datasheet.

    ab151741 has been referenced in 1 publication.

    • Hadjidemetriou I  et al. DLK1/PREF1 marks a novel cell population in the human adrenal cortex. J Steroid Biochem Mol Biol 193:105422 (2019). PubMed: 31265901

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    What pH of antigen retrieval buffer should be?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2013

    Answer

    Thank you for contacting us.

    We recomend citrate buffer at pH 6.0 and EDTA buffer at pH 9.0.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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