Recombinant Anti-VILIP1 antibody [EPR9833] (ab151741)

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Primary - Rabbit Anti-VILIP1 antibody [EPR9833] (ab151741)
WB, IHC-P

Protein - Recombinant Human VILIP1 protein (ab63276)
WB

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Product name

Anti-VILIP1 antibody [EPR9833]
See all VILIP1 primary antibodies
Description

Rabbit monoclonal [EPR9833] to VILIP1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide, corresponding to residues in Human VILIP1 (P62760).
Positive control
- Human fetal brain and Human cerebellum lysates; Human brain and glioma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9833
Isotype

IgG
Research areas

Alternative Versions
- Anti-VILIP1 antibody [EPR9833] - BSA and Azide free (ab188106)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human VILIP1 protein (ab63276)

Our Abpromise guarantee covers the use of ab151741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for ICC/IF or IP.

Function

Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
Tissue specificity

Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level).
Sequence similarities

Belongs to the recoverin family.
Contains 4 EF-hand domains.
Target information above from: UniProt accession P62760 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7447 Human
- Entrez Gene: 26950 Mouse
- Entrez Gene: 24877 Rat
- Omim: 600817 Human
- SwissProt: P62760 Human
- SwissProt: P62761 Mouse
- SwissProt: P62762 Rat
- Unigene: 444212 Human
- Unigene: 27005 Mouse
- Unigene: 105934 Rat
see all
Alternative names
- 21 kDa CABP antibody
- Hippocalcin like protein 3 antibody
- Hippocalcin-like protein 3 antibody
- HLP 3 antibody
- HLP3 antibody
- HPCAL 3 antibody
- HPCAL3 antibody
- HUVISL1 antibody
- Neural visinin-like protein 1 antibody
- Neural visinin-like type 1 protein antibody
- Neurocalcin alpha antibody
- NVL 1 antibody
- NVL1 antibody
- Nvp1 antibody
- OZ1 antibody
- Ratnvp1 antibody
- VILIP antibody
- VILIP-1 antibody
- Visinin antibody
- Visinin like 1 antibody
- Visinin like protein 1 antibody
- Visinin-like protein 1 antibody
- VISL 1 antibody
- VISL1 antibody
- VISL1_HUMAN antibody
- VLP-1 antibody
- Vnsl1 antibody
- Vsnl1 antibody
see all

Western blot - Anti-VILIP1 antibody [EPR9833] (ab151741)

All lanes : Anti-VILIP1 antibody [EPR9833] (ab151741) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab151741 at 1/100 diluton.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling VILIP1 with ab151741 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin embedded Human Retina tissue using ab151741 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9833] (ab151741)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151741 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Hadjidemetriou I et al. DLK1/PREF1 marks a novel cell population in the human adrenal cortex. J Steroid Biochem Mol Biol 193:105422 (2019). Read more (PubMed: 31265901) »

See 1 Publication for this product

Publishing research using ab151741? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

What pH of antigen retrieval buffer should be?

Abcam community

Verified customer

Asked on Feb 22 2013

Answer

Thank you for contacting us.

We recomend citrate buffer at pH 6.0 and EDTA buffer at pH 9.0.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Feb 22 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-VILIP1 antibody [EPR9833] (ab151741)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As