Anti-Villin antibody (ab244291)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Villin aa 700 to the C-terminus.
Database link: P09327 -
Positive control
- IHC-P: Human kidney, small intestine, pancreas, colon, gallbladder and liver tissues. WB: Caco-2 cell lysate. ICC/IF: Caco-2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7429 Human
- Omim: 193040 Human
- SwissProt: P09327 Human
- Unigene: 654595 Human
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Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
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Paraffin-embedded human small intestine tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for Villin (green) using ab244291 at 4 µg/ml in ICC/IF.
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All lanes : Anti-Villin antibody (ab244291) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 97 kDa -
Paraffin-embedded human liver tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human gallbladder tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
Negative control.
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Paraffin-embedded human pancreas tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244291 has not yet been referenced specifically in any publications.