Anti-Villin antibody (ab272876)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Villin (N terminal). The exact sequence is proprietary.
Database link: P09327 -
Positive control
- WB: Mouse kidney tissue extract, H1299 whole cell lysate. IHC-P: NCIN87 xenograft tissue. ICC/IF: Hep3B cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7429 Human
- Entrez Gene: 22349 Mouse
- Omim: 193040 Human
- SwissProt: P09327 Human
- SwissProt: Q62468 Mouse
- Unigene: 654595 Human
- Unigene: 471601 Mouse
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Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
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Anti-Villin antibody (ab272876) at 1/1000 dilution + Mouse kidney tissue at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Paraffin-embedded NCIN87 xenograft tissue stained for Villin using ab272876 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Methanol-fixed Hep3B (human hepatocellular carcinoma cell line) cells stained for Villin using ab272876 at 1/500 dilution in ICC/IF (green).
Co-stained with Hoechst 33342 (blue).
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Anti-Villin antibody (ab272876) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
7.5% SDS PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab272876 has been referenced in 1 publication.
- Chen HY et al. Upregulation of oncogene Activin A receptor type I by Helicobacter pylori infection promotes gastric intestinal metaplasia via regulating CDX2. Helicobacter 26:e12849 (2021). PubMed: 34490965