Product nameAnti-Villin antibody
See all Villin primary antibodies
DescriptionRabbit polyclonal to Villin
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human Villin (N terminal). The exact sequence is proprietary.
Database link: P09327
- WB: Mouse kidney tissue extract, H1299 whole cell lysate. IHC-P: NCIN87 xenograft tissue. ICC/IF: Hep3B cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionCa(2+)-regulated actin-binding protein.
Tissue specificityMajor component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain.
DomainConsists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
Anti-Villin antibody (ab272876) at 1/1000 dilution + Mouse kidney tissue at 50 µg
HRP-conjugated anti-rabbit IgG antibody
Paraffin-embedded NCIN87 xenograft tissue stained for Villin using ab272876 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Methanol-fixed Hep3B (human hepatocellular carcinoma cell line) cells stained for Villin using ab272876 at 1/500 dilution in ICC/IF (green).
Co-stained with Hoechst 33342 (blue).
Anti-Villin antibody (ab272876) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
7.5% SDS PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272876 has not yet been referenced specifically in any publications.