Overview

  • Product name
  • Description
    Rabbit polyclonal to Villin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Villin aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P09327

  • Positive control
    • Small intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab52102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    Ca(2+)-regulated actin-binding protein.
  • Tissue specificity
    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Domain
    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • D2S1471 antibody
    • OTTHUMP00000164145 antibody
    • VIL antibody
    • VIL1 antibody
    • VILI_HUMAN antibody
    • Villin 1 antibody
    • Villin-1 antibody
    • Villin1 antibody
    see all

Images

  • ab52102 at 1/50 dilution staining human Villin in small intestine by Immunohistochemistry, Formalin-fixed Paraffin embedded tissue.

References

This product has been referenced in:

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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