Product nameAnti-Villin antibody
See all Villin primary antibodies
DescriptionRabbit polyclonal to Villin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Villin aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P09327
- Small intestine.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
FunctionCa(2+)-regulated actin-binding protein.
Tissue specificityMajor component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain.
DomainConsists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
This product has been referenced in:
- Yamamoto Y et al. Immunohistochemical characterization of brush cells in the rat larynx. J Mol Histol 49:63-73 (2018). Read more (PubMed: 29196853) »
- Chung I et al. High cell-surface density of HER2 deforms cell membranes. Nat Commun 7:12742 (2016). IF ; Human . Read more (PubMed: 27599456) »