Recombinant
RabMAb

Anti-Villin antibody [EPR3490] (ab109516)

Abreviews (1)Q&A (4)Specific References (2)

Overview

  • Product name
    Anti-Villin antibody [EPR3490]
    See all Villin primary antibodies
  • Description
    Rabbit monoclonal [EPR3490] to Villin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Villin aa 150-250. The exact sequence is proprietary.

  • Positive control
    • Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function
      Ca(2+)-regulated actin-binding protein.
    • Tissue specificity
      Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • Sequence similarities
      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • Domain
      Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Caco-2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 93 kDa
      Observed band size: 95 kDa (why is the actual band size different from the predicted?)

    • Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    • Ozawa T  et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . Read more (PubMed: 26559489) »

    See all 2 Publications for this product

    Publishing research using ab109516? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

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    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Villin antibody [EPR3490]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (iPS cell derived intestinal cells)
    Permeabilization
    Yes - triton x-100
    Specification
    iPS cell derived intestinal cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Apr 26 2018

    I am potentially interested in purchasing anti-villin Ab [erp3490] (ab109516, Epitomic catalog no. 3722-1) for flow cytometry. According to your specs, it reacts with mouse villin, but you only include human data on your specs sheet. Could you send me ...

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    Thank you for contacting us.

    With many products cross reactivity with mouse and rat is determined by western blot only (this is the case for ab109516). If we have only determined the cross reactivity via WB, we will state this information ...

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    Good Afternoon,
    I recently placed an order for a replacement antibody. I received this conformation email (see below) and I just wanted to make sure that we will not be charged for the antibody. Despite the fact that email says we will not there s...

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    Thanks for your enquiry.

    Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order...

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    attempting to stain mouse intestine via IHC-P; this tissue definitely expresses villin and is a pos control sample; another antibodyhas been used to stain this same tissue successfully; primary used at 1:1000 and no staining

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    Estimated delivery date of this replacement vial is August 27 2012. To ch...

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    Dear Tech support, One of our customers wishes to test ab109516 for ICC/IF and Mouse as part of the abreview promotion. Please advise.

    Thank you very much for your interest in ab109516. Unfortunately we would not advise that ab109516 be used in ICC. However we have another antibody in our catalogue that may be suitable for your customer. Anti-Villin antibody (ab97512) is a rab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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