Recombinant Anti-Villin antibody [EPR3490] (ab109516)

Thank you for contacting us.

With many products cross reactivity with mouse and rat is determined by western blot only (this is the case for ab109516). If we have only determined the cross reactivity via WB, we will state this information on the website and datasheet in the "Species Reactivity" section. I have attached an image of our WB data with mouse and rat for ab109516. We recommend the antibody for FC based on the fact that it worked for human samples and the antibody appears to be mouse and rat reactive due to our WB results.

This product has an expected molecular weight of 93 kDa. In the image on the datasheet we do show that this product in near its expected size at around 95 kDa. There are many reasons why a protein may appear to be at a different size than expected.

Markers especially those which are prestained are approximate values. It is difficult to determining precise size based on published weights for calibration markers. Even common proteins may have several slightly different size estimates reported in the literature, depending upon the methods of molecular weight measurement.

Other reasons why a protein may run at an unexpected size include gel type and protein charge. Post translation modifications, cleavage, splice variants and multimers can affect some protein targets as well.

Most proteins will give estimates within a few percent of their actual weight when compared to appropriate calibration markers. If prepared in the same way each time a protein should run reproducibly on a given gel type.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thanks for your enquiry.

Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order. It is now present in our system correctly as "Total goods cost = $0.00".

I have re-sent the order confirmation with the adjusted amount due as $0.00.

Thanks for letting me know of this error. Please let me know if you have any further questions.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

Estimated delivery date of this replacement vial is August 27 2012. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you very much for your interest in ab109516. Unfortunately we would not advise that ab109516 be used in ICC. However we have another antibody in our catalogue that may be suitable for your customer. Anti-Villin antibody (ab97512) is a rabbit polycolnal antibody targeting in the region of 7-256 of Human Villin. Through Blast comparison with mouse villin this region shares 89% homology with the human protein and is therefore predicted to react. As to our knowledge, ab97512 has not been tested in ICC or with mouse protein, I can offer a discount off a future purchase if you buy ab97512 now, test it in mouse with ICC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody of your choice. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab97512 with ICC performed with mouse sample. I will then send a discount code. This code must be issued before purchasing ab97512 so please wait for my reply before ordering. 2. Purchase ab97512 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse with ICC. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab97512 turns out to be unsuitable for mouse ICC, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.