Product nameAnti-Villin antibody [EPR3490]
See all Villin primary antibodies
DescriptionRabbit monoclonal [EPR3490] to Villin
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Villin aa 150-250. The exact sequence is proprietary.
- Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCa(2+)-regulated actin-binding protein.
Tissue specificityMajor component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain.
DomainConsists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : HT-29 lysate
Lane 3 : Caco-2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Benkler C et al. Aggregated SOD1 causes selective death of cultured human motor neurons. Sci Rep 8:16393 (2018). Read more (PubMed: 30401824) »
- Negoro R et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »