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  1. Link

    villin-antibody-epr3490-ab109516.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Villin
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Recombinant
RabMAb

Recombinant Anti-Villin antibody [EPR3490] (ab109516)

  • Datasheet
Reviews (1)Q&A (4)References (3)

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  • Western blot - Anti-Villin antibody [EPR3490] (ab109516)
  • Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)

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  • Datasheet
  • References (3)
  • Protocols

Overview

  • Product name

    Anti-Villin antibody [EPR3490]
    See all Villin primary antibodies
  • Description

    Rabbit monoclonal [EPR3490] to Villin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Villin aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3490
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Villin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Associated products

  • Alternative Versions

    • Anti-Villin antibody [EPR3490] - BSA and Azide free (ab215273)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Villin protein (ab152796)

Applications

Our Abpromise guarantee covers the use of ab109516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Ca(2+)-regulated actin-binding protein.
    • Tissue specificity

      Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • Sequence similarities

      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • Domain

      Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P09327 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7429 Human
      • Entrez Gene: 22349 Mouse
      • Entrez Gene: 316521 Rat
      • Omim: 193040 Human
      • SwissProt: P09327 Human
      • SwissProt: Q62468 Mouse
      • Unigene: 654595 Human
      • Unigene: 471601 Mouse
      • Unigene: 220408 Rat
      see all
    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Caco-2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 93 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Benkler C  et al. Aggregated SOD1 causes selective death of cultured human motor neurons. Sci Rep 8:16393 (2018). Read more (PubMed: 30401824) »
    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    See all 3 Publications for this product

    Publishing research using ab109516? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Villin antibody [EPR3490]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (iPS cell derived intestinal cells)
    Permeabilization
    Yes - triton x-100
    Specification
    iPS cell derived intestinal cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 26 2018

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