Recombinant Anti-Villin antibody [EPR3490] (ab109516)
![Western blot - Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-122430-anti-villin-antibody-epr3490-western-blot.jpg "Western blot - Anti-Villin antibody [EPR3490] (ab109516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3490] to Villin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Villin antibody [EPR3490]
See all Villin primary antibodies -
Description
Rabbit monoclonal [EPR3490] to Villin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3490 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa). |
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
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Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7429 Human
- Entrez Gene: 22349 Mouse
- Entrez Gene: 316521 Rat
- Omim: 193040 Human
- SwissProt: P09327 Human
- SwissProt: Q62468 Mouse
- Unigene: 654595 Human
- Unigene: 471601 Mouse
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Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
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Western blot - Anti-Villin antibody [EPR3490] (ab109516)All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : HT-29 lysate
Lane 3 : Caco-2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-122429-anti-villin-antibody-epr3490-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (ab109516)Intracellular flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
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![Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-3-benefits-of-recombinant-antibodies.png)
Anti-Villin antibody [EPR3490] (ab109516)
Datasheets and documents
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Datasheet download
References (4)
ab109516 has been referenced in 4 publications.
- Kawai K et al. Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies. Mol Ther Methods Clin Dev 17:49-57 (2020). PubMed: 31890740
- Benkler C et al. Aggregated SOD1 causes selective death of cultured human motor neurons. Sci Rep 8:16393 (2018). PubMed: 30401824
- Negoro R et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . PubMed: 26966071
- Ozawa T et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . PubMed: 26559489
