Recombinant
RabMAb

Recombinant Anti-Villin antibody [EPR3490] (ab109516)

Reviews (1)Q&A (4)References (3)

Overview

  • Product name

    Anti-Villin antibody [EPR3490]
    See all Villin primary antibodies

  • Description

    Rabbit monoclonal [EPR3490] to Villin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Villin aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Ca(2+)-regulated actin-binding protein.

    • Tissue specificity

      Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.

    • Sequence similarities

      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.

    • Domain

      Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.

    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      Western blot - Anti-Villin antibody [EPR3490] (ab109516)
      All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Caco-2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 93 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)
      Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Benkler C  et al. Aggregated SOD1 causes selective death of cultured human motor neurons. Sci Rep 8:16393 (2018). Read more (PubMed: 30401824) »
    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    See all 3 Publications for this product

    Publishing research using ab109516? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Villin antibody [EPR3490]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (iPS cell derived intestinal cells)
    Permeabilization
    Yes - triton x-100
    Specification
    iPS cell derived intestinal cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 26 2018

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