Overview

  • Product name

    Anti-Villin antibody [EPR3490] - BSA and Azide free
    See all Villin primary antibodies
  • Description

    Rabbit monoclonal [EPR3490] to Villin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Villin aa 150-250. The exact sequence is proprietary.

  • General notes

    Ab215273 is the carrier-free version of ab109516. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab215273 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Ca(2+)-regulated actin-binding protein.
    • Tissue specificity

      Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • Sequence similarities

      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • Domain

      Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    References

    This product has been referenced in:

    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    • Ozawa T  et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . Read more (PubMed: 26559489) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab215273.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up