Recombinant Anti-Villin antibody [EPR3491(3)] (ab133510)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3491(3)] to Villin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Villin antibody [EPR3491(3)]
See all Villin primary antibodies -
Description
Rabbit monoclonal [EPR3491(3)] to Villin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Villin aa 650-750. The exact sequence is proprietary.
-
Positive control
- Caco-2, HT-29, and Human small intestine lysates; Human colon adenocarcinoma tissue, colon and kidney tissues
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3491(3) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 93 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/10000 - 1/50000. Predicted molecular weight: 93 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 7429 Human
- Omim: 193040 Human
- SwissProt: P09327 Human
- Unigene: 654595 Human
-
Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling Villin with ab133510 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-Villin antibody [EPR3491(3)] (ab133510) at 1/10000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : Human small intestine tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 93 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Villin with ab133510 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Villin with ab133510 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab133510 has been referenced in 1 publication.
- Derakhshan MH et al. In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity. Gut 64:1705-14 (2015). PubMed: 25753030