Overview

  • Product name

    Anti-Villin antibody [EPR3491(3)]
    See all Villin primary antibodies
  • Description

    Rabbit monoclonal [EPR3491(3)] to Villin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Villin aa 650-750. The exact sequence is proprietary.

  • Positive control

    • Caco-2, HT-29, and Human small intestine lysates; Human colon adenocarcinoma tissue, colon and kidney tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Ca(2+)-regulated actin-binding protein.
    • Tissue specificity

      Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • Sequence similarities

      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • Domain

      Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling Villin with ab133510 at 1/100 dilution.
    • All lanes : Anti-Villin antibody [EPR3491(3)] (ab133510) at 1/10000 dilution

      Lane 1 : CaCo-2 lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 93 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Villin with ab133510 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Villin with ab133510 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Derakhshan MH  et al. In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity. Gut 64:1705-14 (2015). Read more (PubMed: 25753030) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133510.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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