Overview

  • Product name

    Anti-Villin antibody [M245]
    See all Villin primary antibodies
  • Description

    Mouse monoclonal [M245] to Villin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control

    • Adult mouse kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab86704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB 1/1000. Predicted molecular weight: 93 kDa.

Target

  • Function

    Ca(2+)-regulated actin-binding protein.
  • Tissue specificity

    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Domain

    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • D2S1471 antibody
    • OTTHUMP00000164145 antibody
    • VIL antibody
    • VIL1 antibody
    • VILI_HUMAN antibody
    • Villin 1 antibody
    • Villin-1 antibody
    • Villin1 antibody
    see all

Images

  • Anti-Villin antibody [M245] (ab86704) at 1/250 dilution + Adult mouse kidney lysate

    Predicted band size: 93 kDa



    Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.

References

ab86704 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and below)to mouse to expect any cross-reactivity. Unfortunately, this is also true for the other antibodies we offer against these targets too.

ab86704 is already tested and guaranteed for mouse and WB. Please reassure your customer that the Abpromise guarantee will apply to this antibody in their experiment.

DISCOUNT CODEs:

ab68715: xxxxx


ab86177: xxxxx


ab21156: xxxxx

Expiration date:17. May 2012

I am very pleased to hear your customer would like to accept our offer and test ab68715 and ab86177 and ab21156 in mouse.

The codes will give your customer 1 freeprimary antibody eachbefore the expiration date.

To redeem this offer, please submit an Abreview eachformouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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