Overview

  • Product name

    Anti-Villin antibody [VIL1/1314]
    See all Villin primary antibodies
  • Description

    Mouse monoclonal [VIL1/1314] to Villin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
    Database link: P09327

  • Positive control

    • A549, HepG2 and HCT116 cell lysates; Human colon and rectum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ca(2+)-regulated actin-binding protein.
  • Tissue specificity

    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Domain

    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • D2S1471 antibody
    • OTTHUMP00000164145 antibody
    • VIL antibody
    • VIL1 antibody
    • VILI_HUMAN antibody
    • Villin 1 antibody
    • Villin-1 antibody
    • Villin1 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Colon tissue labeling Villin with ab218331 at 0.5µg/ml.

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Rectum tissue labeling Villin with ab218331 at 0.5µg/ml.

  • All lanes : Anti-Villin antibody [VIL1/1314] (ab218331) at 2 µg/ml

    Lane 1 : A549 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HCT116 cell lysate

    Predicted band size: 93 kDa

References

ab218331 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rabbit Tissue lysate - whole (intestinal epithelium)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
intestinal epithelium
Blocking step
BSA / Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 11 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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