Anti-Villin antibody [VIL1/1314] (ab218331)

Reviews (1) Submit a question

Overview

  • Product name

    Anti-Villin antibody [VIL1/1314]
    See all Villin primary antibodies

  • Description

    Mouse monoclonal [VIL1/1314] to Villin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
    Database link: P09327

  • Positive control

    • A549, HepG2 and HCT116 cell lysates; Human colon and rectum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ca(2+)-regulated actin-binding protein.

  • Tissue specificity

    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.

  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.

  • Domain

    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Colon tissue labeling Villin with ab218331 at 0.5µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Rectum tissue labeling Villin with ab218331 at 0.5µg/ml.

    • Western blot - Anti-Villin antibody [VIL1/1314] (ab218331)
      Western blot - Anti-Villin antibody [VIL1/1314] (ab218331)
      All lanes : Anti-Villin antibody [VIL1/1314] (ab218331) at 2 µg/ml

      Lane 1 : A549 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HCT116 cell lysate

      Predicted band size: 93 kDa

    References

    ab218331 has not yet been referenced specifically in any publications.

    Publishing research using ab218331? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Western blot abreview for Anti-Villin antibody [VIL1/1314]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (intestinal epithelium)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    intestinal epithelium
    Blocking step
    BSA / Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 11 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up