Anti-Villin antibody [VIL1/1314] (ab218331)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
  • Western blot - Anti-Villin antibody [VIL1/1314] (ab218331)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)

Key features and details

  • Mouse monoclonal [VIL1/1314] to Villin
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Villin antibody [VIL1/1314]
    See all Villin primary antibodies

  • Description

    Mouse monoclonal [VIL1/1314] to Villin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
    Database link: P09327

  • Positive control

    • WB: A549, HepG2 and HCT116 cell lysates. IHC-P: Human colon and rectum tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ca(2+)-regulated actin-binding protein.

  • Tissue specificity

    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.

  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.

  • Domain

    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Colon tissue labeling Villin with ab218331 at 0.5µg/ml.

    • Western blot - Anti-Villin antibody [VIL1/1314] (ab218331)
      Western blot - Anti-Villin antibody [VIL1/1314] (ab218331)
      All lanes : Anti-Villin antibody [VIL1/1314] (ab218331) at 2 µg/ml

      Lane 1 : A549 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HCT116 cell lysate

      Predicted band size: 93 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Rectum tissue labeling Villin with ab218331 at 0.5µg/ml.

    References (1)

    Publishing research using ab218331? Please let us know so that we can cite the reference in this datasheet.

    ab218331 has been referenced in 1 publication.

    • Kawai K  et al. Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies. Mol Ther Methods Clin Dev 17:49-57 (2020). PubMed: 31890740

    Customer reviews and Q&As

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    Western blot abreview for Anti-Villin antibody [VIL1/1314]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (intestinal epithelium)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    intestinal epithelium
    Blocking step
    BSA / Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 11 2018

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