Anti-Villin antibody [VIL1/1314] (ab218331)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Villin antibody [VIL1/1314]
See all Villin primary antibodies -
Description
Mouse monoclonal [VIL1/1314] to Villin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
Database link: P09327 -
Positive control
- A549, HepG2 and HCT116 cell lysates; Human colon and rectum tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
VIL1/1314 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab218331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa. | |
IHC-P | Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7429 Human
- Omim: 193040 Human
- SwissProt: P09327 Human
- Unigene: 654595 Human
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Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Colon tissue labeling Villin with ab218331 at 0.5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (ab218331)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human Rectum tissue labeling Villin with ab218331 at 0.5µg/ml.
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All lanes : Anti-Villin antibody [VIL1/1314] (ab218331) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HCT116 cell lysate
Predicted band size: 93 kDa
Datasheets and documents
References
ab218331 has not yet been referenced specifically in any publications.