• Product name

  • Description

    Goat polyclonal to Vimentin
  • Host species

  • Specificity

    No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.

  • Positive control

  • General notes

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.



Our Abpromise guarantee covers the use of ab11256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.
IHC-Fr 1/20.
WB 1/50. Detects a band of approximately 58 kDa.

The antibody specifically stains the vimentin band using total cell or tissue extracts.

ICC Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.


  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all


  • ii Panels i-iii, TGF-ß1 induces EMT-type changes in primary hepatocytes. Immunofluorescence staining for E-cadherin (green) and vimentin (red) in primary hepatocytes were evaluated by confocal laser microscopy. DAPI stained nuclei blue. There was apparent near loss of cell membrane-bound E-cadherin in TGF-ß1-treated cells in a time-dependent manner. Diffuse vimentin staining was observed in control cells, and TGF-ß1-treated hepatocytes exhibited a fibrillar vimentin pattern most noted at 48 and 72 h. Also note that TGF-ß1 caused hepatocytes to assume a spindle-shaped morphology, which was not observed in controls. Scale bar, 50 µm.
  • Immunocytochemistry/ Immunofluorescence analysis of mouse sertoli cells labeling Vimentin with ab11256 at 1/20 dilution. Cells were fixed with methanol and blocked with 5% serum for 20 minutes at 25 °C. A polyclonal Donkey anti-goat Dylight® 488 was used as the secondary antibody at 1/400 dilution.

    See Abreview


This product has been referenced in:

  • Lee J  et al. Development of a patient-derived xenograft model of glioblastoma via intravitreal injection in mice. Exp Mol Med 51:43 (2019). Read more (PubMed: 30992429) »
  • Nakanishi H  et al. Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias. Front Physiol 10:818 (2019). Read more (PubMed: 31316396) »
See all 21 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Human Cell (breast cancer cell lines)
breast cancer cell lines
Yes - 0.3% Triton X-100 in TBS

Abcam user community

Verified customer

Submitted Dec 18 2014

Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Human Cell lysate - whole cell (Breast cancer cell lines)
Breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 10 2014


Yes this antibody has been tested on human paraffin embedded tissue followed by HIER.

The following publication used our antibody in this application on human placenta:

Lockwood CJ et al. Progestin and thrombin regulate tissue factor expression in human term decidual cells. J Clin Endocrinol Metab 94:2164-70 (2009).

Read More


The antigen retrieval was performed using 0.1% Trypsin Enzyme incubation for 15 - 25 min. The enzyme was incubated at a temperature of 37°C

Read More
Immunocytochemistry/ Immunofluorescence
Mouse Cell (sertoli cells)
sertoli cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Elad Fadlon

Verified customer

Submitted Aug 06 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Dog Tissue sections (Heart)
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 21 2006

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