Anti-Vimentin antibody (ab137321)

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Overview

  • Product name

    Anti-Vimentin antibody
    See all Vimentin primary antibodies

  • Description

    Rabbit polyclonal to Vimentin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Guinea pig, Cow, Dog, Chimpanzee

  • Immunogen

    Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary.
    Database link: P08670

  • Positive control

    • WB: HEK-293T, HeLa and NIH/3T3 whole cell lysates. U87-MG, SK-N-SH. IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IHC-P: Adult mouse retina tissue. U373 xenograft tissue and RT2 rat xenograft tissue. IP: HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab137321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
IP Use at an assay dependent concentration.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • Involvement in disease

    Cataract 30

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.

  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Western blot - Anti-Vimentin antibody (ab137321)
    Western blot - Anti-Vimentin antibody (ab137321)
    All lanes : Anti-Vimentin antibody (ab137321) at 1/50000 dilution

    Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
    Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts
    Lane 3 : IMR32 whole cell extracts
    Lane 4 : SK-N-AS whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 54 kDa



    10% SDS-PAGE gel

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.

    Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
    Blue: Fluoroshield with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

  • Immunoprecipitation - Anti-Vimentin antibody (ab137321)
    Immunoprecipitation - Anti-Vimentin antibody (ab137321)

    Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 µg of ab137321.

    Lane 1: HeLa whole cell extracts (Input).

    Lane 2: Control IgG in HeLa whole cell extracts.

    Lane 3: Vimentin IP in HeLa whole cell extracts.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.

  • Western blot - Anti-Vimentin antibody (ab137321)
    Western blot - Anti-Vimentin antibody (ab137321)
    Anti-Vimentin antibody (ab137321) at 1/3000 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    7.5% SDS PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)

    Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.

  • Western blot - Anti-Vimentin antibody (ab137321)
    Western blot - Anti-Vimentin antibody (ab137321)
    All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

    Predicted band size: 54 kDa



    10% SDS PAGE

  • Western blot - Anti-Vimentin antibody (ab137321)
    Western blot - Anti-Vimentin antibody (ab137321)
    Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS Page
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.

References

This product has been referenced in:

  • Bai J  et al. lncRNA SNHG1 cooperated with miR-497/miR-195-5p to modify epithelial-mesenchymal transition underlying colorectal cancer exacerbation. J Cell Physiol 235:1453-1468 (2020). Read more (PubMed: 31276207) »
  • Xi X  et al. MicroRNA-204-3p represses colon cancer cells proliferation, migration, and invasion by targeting HMGA2. J Cell Physiol 235:1330-1338 (2020). Read more (PubMed: 31286521) »
See all 56 Publications for this product

Publishing research using ab137321? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I’m looking to characterise a cell line I use to confirm it has a myofibroblast phenotype. Typically myofibroblasts test positive for Vimentin and Smooth muscle actin but are negative for desmin. I am preferably not looking to purchase large amounts of either antibody, is there any way I can purchase smaller amounts than the advertised quantities?

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Answer


Unfortunately, if we do not have a link on the datasheet towards a smaller size (which is actually quite rare), then we cannot offer you a different size than the one we have in our catalog.

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Question

Dear Abcam,



Hi, I'm trying to detect fibrosis on a rat heart tissue that has undergone ischemia reperfusion injury.

I wanted to know if your anti-vimentin antibody ab137321 works on rat heart tissue for IHC-P and WB.

Some antibodies that say they cross react with rat, did not react with the heart so I wanted to make sure this antibody will work

Also, for anti-F4/80 ab74383, it says it works with WB but does it also work with IHC-P?

Do you have any references, articles that have stated use of these antibodies on rat heart tissue?



Your assistance will be much appreciated!

Thank you



Kind regards,

Read More
Answer

Thank you for your enquiry and your interest in our products.

I can confirm that both antibodies have been tested for cross-reaction with rat so they recognize this species. However, neither of them have been specifically used on rat hear tissue sections.

It would be worth checking some published papers using PubMed if F4/80 and Vimentin are widely used as good markers for investigating fibrosis on heart tissue. If these proteins are expressed at detectable levels, then ab74383 and ab137321 can be used in this study.

If you need any further assistance in the future, please do not hesitate to contact me.

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