Anti-Vimentin antibody (ab137321)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Vimentin antibody
See all Vimentin primary antibodies -
Description
Rabbit polyclonal to Vimentin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Guinea pig, Cow, Dog, Chimpanzee
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Immunogen
Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary.
Database link: P08670 -
Positive control
- WB: HEK-293T, HeLa and NIH/3T3 whole cell lysates. U87-MG, SK-N-SH. IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IHC-P: Adult mouse retina tissue. U373 xenograft tissue and RT2 rat xenograft tissue. IP: HeLa whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P | (1) |
1/100 - 1/1000.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 420519 Chicken
- Entrez Gene: 493952 Chimpanzee
- Entrez Gene: 280955 Cow
- Entrez Gene: 477991 Dog
- Entrez Gene: 100170760 Guinea pig
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
see all -
Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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Western blot - Anti-Vimentin antibody (ab137321)All lanes : Anti-Vimentin antibody (ab137321) at 1/50000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 whole cell extracts
Lane 4 : SK-N-AS whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa10% SDS-PAGE gel
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Immunoprecipitation - Anti-Vimentin antibody (ab137321)Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 µg of ab137321.
Lane 1: HeLa whole cell extracts (Input).
Lane 2: Control IgG in HeLa whole cell extracts.
Lane 3: Vimentin IP in HeLa whole cell extracts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
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Western blot - Anti-Vimentin antibody (ab137321)Anti-Vimentin antibody (ab137321) at 1/3000 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 54 kDa7.5% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
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Western blot - Anti-Vimentin antibody (ab137321)All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 54 kDa10% SDS PAGE
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Western blot - Anti-Vimentin antibody (ab137321)Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS Page -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (110)
ab137321 has been referenced in 110 publications.
- Bai J et al. lncRNA SNHG1 cooperated with miR-497/miR-195-5p to modify epithelial-mesenchymal transition underlying colorectal cancer exacerbation. J Cell Physiol 235:1453-1468 (2020). PubMed: 31276207
- Xi X et al. MicroRNA-204-3p represses colon cancer cells proliferation, migration, and invasion by targeting HMGA2. J Cell Physiol 235:1330-1338 (2020). PubMed: 31286521
- Zhang NN et al. Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase. Sci Rep 10:439 (2020). PubMed: 31949205
- Feng J & Wang T MicroRNA-873 serves a critical role in human cervical cancer proliferation and metastasis via regulating glioma-associated oncogene homolog 1. Exp Ther Med 19:1243-1250 (2020). PubMed: 32010295
- Wang C et al. Blocking the autocrine regulatory loop of Gankyrin/STAT3/CCL24/CCR3 impairs the progression and pazopanib resistance of clear cell renal cell carcinoma. Cell Death Dis 11:117 (2020). PubMed: 32051393