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    vimentin-antibody-ab24525.pdf

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Anti-Vimentin antibody (ab24525)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (80)

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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)
  • Western blot - Anti-Vimentin antibody (ab24525)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)

Key features and details

  • Chicken polyclonal to Vimentin
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Vimentin antibody
    See all Vimentin primary antibodies
  • Description

    Chicken polyclonal to Vimentin
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Vimentin.
    Database link: P08670

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    IgY fraction
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Vimentin antibody [LN-6] (ab230171)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Related Products

    • Human Vimentin Profiling ELISA Kit (ab173190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
WB (1)
1/10000.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/10000.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7431 Human
    • Entrez Gene: 22352 Mouse
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P08670 Human
    • SwissProt: P20152 Mouse
    • SwissProt: P31000 Rat
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 268000 Mouse
    • Unigene: 2710 Rat
    see all
  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)

    Immunocytochemistry/ Immunofluorescence analysis of neuron/glial cultures labeling Vimentin with ab24525 (green) and GFAP with ab7260 (red). Vimentin is the sole cytoplasmic intermediate filament subunit expressed in fibroblasts, microglial and endothelial cells. The flattened cells in the middle of the image which appear green are fibroblasts. Astrocytes may express primarily GFAP, or both GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)

    Rat cerebral cortex cultures stained with chicken antibody to vimentin ab24525 (green) and rabbit antibody to GFAP (red). Note flattened fibroblastic cells are mostly green (i.e. vimentin positive, GFAP negative), while clearly astrocytic cells, express both vimentin and GFAP and therefore appear golden or orange. Certain other cells express predominantly GFAP and therefore appear red.

     

  • Western blot - Anti-Vimentin antibody (ab24525)
    Western blot - Anti-Vimentin antibody (ab24525)

    Western blot of Rat whole brain extract,HeLa,SH-SY5Y,HEK293, and NIH/3T3 cells probed with ab24525,showing a single strong band at ~ 50kDa.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab24525)This image is a courtesy of Anonymous Abreview

    ab24525 staining Vimentin in rat smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Abcam`s ab6875, goat anti-chicken IgY Texas Red was used as secondary antibody at 1/400 dilution.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (80)

Publishing research using ab24525? Please let us know so that we can cite the reference in this datasheet.

ab24525 has been referenced in 80 publications.

  • Hu Y  et al. miR-491-3p is Downregulated in Retinoblastoma and Inhibit Tumor Cells Growth and Metastasis by Targeting SNN. Biochem Genet 59:453-474 (2021). PubMed: 33098307
  • Anand A  et al. Elimination of NF-?B signaling in Vimentin+ stromal cells attenuates tumorigenesis in a mouse model of Barrett's Esophagus. Carcinogenesis 42:405-413 (2021). PubMed: 33068426
  • Lu N  et al. miRNA-490-3p promotes the metastatic progression of invasive ductal carcinoma. Oncol Rep 45:706-716 (2021). PubMed: 33416185
  • Zhu G  et al. P53/miR-154 Pathway Regulates the Epithelial-Mesenchymal Transition in Glioblastoma Multiforme Cells by Targeting TCF12. Neuropsychiatr Dis Treat 17:681-693 (2021). PubMed: 33664574
  • Makarava N  et al. The degree of astrocyte activation is predictive of the incubation time to prion disease. Acta Neuropathol Commun 9:87 (2021). PubMed: 33980286
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 22 2021

IHC - Wholemount abreview for Anti-Vimentin antibody

Poor
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Cornea)
Specification
Cornea
Read More

Abcam user community

Verified customer

Submitted Aug 23 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin (foreskin))
Permeabilization
No
Specification
Human skin (foreskin)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Unfixed
Read More

Abcam user community

Verified customer

Submitted Mar 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human skin (foreskin))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 8.3
Permeabilization
Yes - 5 minutes PBS-Triton 0.2%
Specification
human skin (foreskin)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 27 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.1% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 28 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E17.5 Embryo Heart Sections - 10um thick)
Permeabilization
Yes - 0.5% Tween-20
Specification
E17.5 Embryo Heart Sections - 10um thick
Blocking step
5% Secondary specific serum + 1% BSA + 0.5% Tween-20 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 17 2017

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 12 2014

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Aug 28 2013

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 17 2013

Western blot abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac fibroblasts)
Loading amount
4 µg
Specification
Cardiac fibroblasts
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 04 2013

1-10 of 11 Abreviews or Q&A

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