Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (smooth muscle cells from mesenteric artery)
Specification
smooth muscle cells from mesenteric artery
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Other product details

Dilution
1/300
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PSS with 2%BSA and 0.3% Triton X-100

Secondary antibody

Dilution
1/400

Additional data

Additional Notes
AB was aliquoted and stored at -20°C
Fixation in 4% paraformaldehyde in physiological saline solution (PSS) 4 min at 4°C,
AB works fine in dilution range of 1:200 - 1:500

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Submitted Nov 17 2009

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