Western blot abreview for Anti-Vimentin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac fibroblasts)
Loading amount
4 µg
Specification
Cardiac fibroblasts
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Dilution
1/2500

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 57 kDa

Additional data

Additional Notes
Excellent antibody for detection of mouse Vimentin by Western blot. Do not give unspecific bands.

Abcam user community

Verified customer

Submitted Feb 04 2013

Sign up