Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%donkey serum 0.3%tritonx-100 in kpbs

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Immunoresearch donkey anti-chicken
Host species: Donkey
Clonality: Polyclonal
Conjugation: Aminomethylcoumarin Acetate
Dilution
1/500

Additional data

Additional Notes
Free floating section. Very good axonal labeling in the tanycytes.

Abcam user community

Verified customer

Submitted Dec 12 2014

Sign up