Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E17.5 Embryo Heart Sections - 10um thick)
Permeabilization
Yes - 0.5% Tween-20
Specification
E17.5 Embryo Heart Sections - 10um thick
Blocking step
5% Secondary specific serum + 1% BSA + 0.5% Tween-20 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Anti-Chicken IgG (H+L) Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

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Submitted May 17 2017

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