Key features and details
- Rabbit polyclonal to Vimentin - C-terminal
- Suitable for: IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-Vimentin antibody - C-terminal
See all Vimentin primary antibodies
DescriptionRabbit polyclonal to Vimentin - C-terminal
Tested applicationsSuitable for: IHC - Wholemount, WBmore details
Species reactivityReacts with: Zebrafish
Synthetic peptide within Zebrafish Vimentin (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9YHX5
- WB: Whole zebrafish extract. IHC-Wm: Zebrafish embryo (1 and 2 days post-fertilization).
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||1/50 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Involvement in diseaseCataract 30
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
FormVimentin is found in connective tissue and in the cytoskeleton.
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Anti-Vimentin antibody - C-terminal (ab229622) at 1/1000 dilution + Whole zebrafish extract at 30 µg
Predicted band size: 53 kDa
10% SDS-PAGE gel.
2 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/50 dilution in wholemount immunohistochemical analysis.
2 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/100 dilution in wholemount immunohistochemical analysis.
1 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/50 dilution in wholemount immunohistochemical analysis.
1 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/100 dilution in wholemount immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229622 has not yet been referenced specifically in any publications.