Overview

  • Product name

    Anti-Vimentin antibody - C-terminal
    See all Vimentin primary antibodies
  • Description

    Rabbit polyclonal to Vimentin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC - Wholemount, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Synthetic peptide within Zebrafish Vimentin (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q9YHX5

  • Positive control

    • WB: Whole zebrafish extract. IHC-Wm: Zebrafish embryo (1 and 2 days post-fertilization).

Properties

Applications

Our Abpromise guarantee covers the use of ab229622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/50 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Anti-Vimentin antibody - C-terminal (ab229622) at 1/1000 dilution + Whole zebrafish extract at 30 µg

    Predicted band size: 53 kDa



    10% SDS-PAGE gel.

  • 2 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/50 dilution in wholemount immunohistochemical analysis.

  • 2 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/100 dilution in wholemount immunohistochemical analysis.

  • 1 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/50 dilution in wholemount immunohistochemical analysis.

  • 1 day-post-fertilization zebrafish embryo stained for Vimentin using ab229622 at 1/100 dilution in wholemount immunohistochemical analysis.

References

ab229622 has not yet been referenced specifically in any publications.

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