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  1. Link

    vimentin-antibody-cytoskeleton-marker-ab45939.pdf

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Validated using a knockout cell line

Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

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Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

Key features and details

  • Rabbit polyclonal to Vimentin - Cytoskeleton Marker
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Vimentin antibody - Cytoskeleton Marker
    See all Vimentin primary antibodies
  • Description

    Rabbit polyclonal to Vimentin - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Vimentin.

    Read Abcam's proprietary immunogen policy (Peptide available as ab46156)
  • Positive control

    • Purchase matching WB positive control:Recombinant Human Vimentin protein
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Vimentin antibody [LN-6] (ab230171)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Vimentin peptide (ab46156)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Human Vimentin Profiling ELISA Kit (ab173190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (9)
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF (2)
Use a concentration of 1 µg/ml.
Flow Cyt (1)
Use 0.01-0.1µg for 106 cells.
Notes
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use 0.01-0.1µg for 106 cells.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 420519 Chicken
    • Entrez Gene: 493952 Chimpanzee
    • Entrez Gene: 280955 Cow
    • Entrez Gene: 7431 Human
    • Entrez Gene: 22352 Mouse
    • Entrez Gene: 100522394 Pig
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P09654 Chicken
    • SwissProt: Q5R1W8 Chimpanzee
    • SwissProt: P48616 Cow
    • SwissProt: P08670 Human
    • SwissProt: P20152 Mouse
    • SwissProt: P02543 Pig
    • SwissProt: P31000 Rat
    • SwissProt: Q9MZA9 Sheep
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 268000 Mouse
    • Unigene: 2710 Rat
    see all
  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    All lanes : Anti-Vimentin antibody - Cytoskeleton Marker (ab45939) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole cell lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole cell lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : HUVEC (Human umbilical vein endothelial cell line) Whole cell lysate
    Lane 8 : A431 (Human epithelial carcinoma cell line) Whole cell lysate
    Lane 9 : Daudi (Human B lymphoblast) Whole cell lysate
    Lane 10 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat Anti-Rabbit at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab45939 overnight at 4°C. The membrane was then blocked for an hour using LI-COR® blocking buffer before being incubated with ab92547 overnight at 4°C. Antibody binding was detected using the IRDye® 800CW Goat Anti-Rabbit secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Odyssey® CLx Imaging System.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    ab45939 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab45939 at 1μg/ml dilution and ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with ab150081 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    ab45939 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab45939 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150120, Goat polyclonal Secondary Antibody to Mouse at 1/1000 dilution (shown in pseudocolour red) and ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    IHC image of ab45939 staining Vimentin in human kidney formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45939, 0.1μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    ab45939 staining Vimentin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab45939 at 1μg/ml (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    ab45939 staining Vimentin in NIH3T3 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab45939 at 1μg/ml (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    Immunohistochemical detection (formaldehyde/paraffin-embedded sections) of vimentin protein using vimentin antibody - Neural Stem Cell Marker (ab45939) on human ovarian carcinoma sections. Antigen retrieval step:Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. Ab45939 was used at 1/700 for 1 hour. Secondary Antibody: Biotin-conjugated anti rabbit IG (1/300).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)Image courtesy of Mike Forbes by Abreview.
    ab45939 staining Vimentin in murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/2100 dilution for 18 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    All lanes : Anti-Vimentin antibody - Cytoskeleton Marker (ab45939) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    All lanes : Anti-Vimentin antibody - Cytoskeleton Marker (ab45939) at 1 µg/ml

    Lane 1 : Recombinant Human Vimentin protein (ab73843) at 0.1 µg
    Lane 2 : Recombinant Human Vimentin protein (ab73843) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 10 seconds


    Ab45939 recognizes full length recombinant Human vimentin (ab73843) which has an expected molecular weight of 54 kDa.
  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    Overlay histogram showing MDA-MB-231 cells stained with ab45939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45939, 0.01μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    Overlay histogram showing NIH3T3 cells stained with ab45939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45939, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)
    Flow Cytometry - Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)

    Overlay histogram showing SV40LT-SMC cells stained with ab45939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45939, 0.01μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (157)

Publishing research using ab45939? Please let us know so that we can cite the reference in this datasheet.

ab45939 has been referenced in 157 publications.

  • Li SL  et al. SLC12A8 plays a key role in bladder cancer progression and EMT. Open Med (Wars) 16:58-67 (2021). PubMed: 33364434
  • Pustovalova M  et al. The CD44high Subpopulation of Multifraction Irradiation-Surviving NSCLC Cells Exhibits Partial EMT-Program Activation and DNA Damage Response Depending on Their p53 Status. Int J Mol Sci 22:N/A (2021). PubMed: 33673439
  • Zhu H  et al. KAT8/MOF-Mediated Anti-Cancer Mechanism of Gemcitabine in Human Bladder Cancer Cells. Biomol Ther (Seoul) 29:184-194 (2021). PubMed: 33041265
  • Fang L  et al. Naoxintong accelerates diabetic wound healing by attenuating inflammatory response. Pharm Biol 59:252-261 (2021). PubMed: 33684026
  • Pantazopoulou V  et al. Hypoxia-Induced Reactivity of Tumor-Associated Astrocytes Affects Glioma Cell Properties. Cells 10:N/A (2021). PubMed: 33802060
View all Publications for this product

Customer reviews and Q&As

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1-10 of 17 Abreviews

IHC - Wholemount abreview for Anti-Vimentin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Rat Tissue (Testis, Seminiferous Tubules)
Specification
Testis, Seminiferous Tubules
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Brad Hansen

Verified customer

Submitted Oct 07 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Le Zhen

Verified customer

Submitted Aug 22 2019

Flow Cytometry abreview for Anti-Vimentin antibody - Cytoskeleton Marker

Average
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rabbit Cell (adipose)
Permeabilization
No
Specification
adipose
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 20 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (salivary gland)
Permeabilization
No
Specification
salivary gland
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 31 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Blockaid molecular probes as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
None
Sample
Sheep Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Zinc Salts
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Helen Todd

Verified customer

Submitted Aug 18 2014

Western blot abreview for Anti-Vimentin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12% page)
Sample
Human Cell lysate - whole cell (hep3b and L3.6PL)
Specification
hep3b and L3.6PL
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 16 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Sample
Human Cell (human ascites- pancreatic cancer cell line)
Specification
human ascites- pancreatic cancer cell line
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 14 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Vimentin antibody - Neural Stem Cell Marker

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sophie Pezet

Verified customer

Submitted Mar 20 2012

Western blot abreview for Anti-Vimentin antibody - Neural Stem Cell Marker

Below Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Recombinant protein (recombinant vimentin)
Loading amount
0.01 µg
Specification
recombinant vimentin
Gel Running Conditions
Reduced Denaturing
Blocking step
Amersham ECL Blocking Agent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 18 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody - Neural Stem Cell Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mike Forbes

Verified customer

Submitted Feb 09 2011

1-10 of 17 Abreviews

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