Key features and details
- Mouse monoclonal [RV202] to Vimentin (FITC)
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Goat, Chicken, Hamster, Dog, Human, Pig, Xenopus laevis, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-Vimentin antibody [RV202] (FITC)
See all Vimentin primary antibodies
DescriptionMouse monoclonal [RV202] to Vimentin (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Goat, Chicken, Hamster, Dog, Human, Pig, Xenopus laevis, Monkey
Does not react with: Zebrafish
Tissue, cells or virus corresponding to Cow Vimentin. The immunogen is cytoskeletal vimentin extract of calf lens.
Database link: P08670
- IHC-Fr: Swine colon tissue. Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab128507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/10 - 1/200.|
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Involvement in diseaseCataract 30
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
FormVimentin is found in connective tissue and in the cytoskeleton.
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ab128507, at 1/200 dilution, staining Vimentin in a frozen section of Swine colon tissue by Immunohistochemistry.
Overlay histogram showing HeLa cells stained with ab128507 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128507, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 1μg/1x106 cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab128507 has been referenced in 4 publications.
- Su L et al. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis. Sci Rep 9:5747 (2019). PubMed: 30952998
- Theodoraki MN et al. Plasma-derived Exosomes Reverse Epithelial-to-Mesenchymal Transition after Photodynamic Therapy of Patients with Head and Neck Cancer. Oncoscience 5:75-87 (2018). PubMed: 29854876
- Vishnyakova PA et al. Mitochondrial role in adaptive response to stress conditions in preeclampsia. Sci Rep 6:32410 (2016). PubMed: 27573305
- Yew KH et al. Epimorphin-Induced MET Sensitizes Ovarian Cancer Cells to Platinum. PLoS One 8:e72637 (2013). PubMed: 24039787