Anti-Vimentin antibody [RV203] (ab8979)

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Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)

Key features and details

  • Mouse monoclonal [RV203] to Vimentin
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Vimentin antibody [RV203]
    See all Vimentin primary antibodies

  • Description

    Mouse monoclonal [RV203] to Vimentin

  • Host species

    Mouse

  • Specificity

    This antibody reacts exclusively with vimentin which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse

  • Immunogen

    Recombinant full length protein corresponding to Bovine Vimentin. Cytoskeletal vimentin extract of calf lens.
    Database link: P08670

  • Positive control

  • General notes

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr
Use at an assay dependent concentration. Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Notes
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr
Use at an assay dependent concentration. Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • Involvement in disease

    Cataract 30

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Form

      Vimentin is found in connective tissue and in the cytoskeleton.

    • Alternative names

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)

      Immunohistochemistry with ab8979 on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      IHC image of ab8979 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8979, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry on frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry on paraffin section of human colon
    • Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)
      Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)
      Overlay histogram showing HeLa cells stained with ab8979 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8979, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

    References (22)

    Publishing research using ab8979? Please let us know so that we can cite the reference in this datasheet.

    ab8979 has been referenced in 22 publications.

    • Wencong M  et al. FOXK1 Promotes Proliferation and Metastasis of Gallbladder Cancer by Activating AKT/mTOR Signaling Pathway. Front Oncol 10:545 (2020). PubMed: 32363163
    • Deng D  et al. Long non-coding RNA SNHG16 regulates cell behaviors through miR-542-3p/HNF4a axis via RAS/RAF/MEK/ERK signaling pathway in pediatric neuroblastoma cells. Biosci Rep 40:N/A (2020). PubMed: 32412051
    • Borges AA  et al. Isolation, characterization, and cryopreservation of collared peccary skin-derived fibroblast cell lines. PeerJ 8:e9136 (2020). PubMed: 32547858
    • Guo R  et al. Long non-coding RNA PRNCR1 modulates non-small cell lung cancer cell proliferation, apoptosis, migration, invasion, and EMT through PRNCR1/miR-126-5p/MTDH axis. Biosci Rep 40:N/A (2020). PubMed: 31912882
    • Shen J  et al. Membrane nanotubes facilitate the propagation of inflammatory injury in the heart upon overactivation of the ß-adrenergic receptor. Cell Death Dis 11:958 (2020). PubMed: 33161415
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [RV203]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Permeabilization
    Yes - Saponin 0.5%
    Specification
    A549
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 14 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [RV203]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Reacts exclusively with vimentin)
    Specification
    Reacts exclusively with vimentin
    Fixative
    Methanol
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% BSA
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 24 2007

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