• Product name
    Anti-Vimentin antibody [VI-10]
    See all Vimentin primary antibodies
  • Description
    Mouse monoclonal [VI-10] to Vimentin
  • Host species
  • Specificity
    The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Vimentin.

  • Positive control



Our Abpromise guarantee covers the use of ab20346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.


  • Function
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease
    Cataract 30
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Form
    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all


  • All lanes : Anti-Vimentin antibody [VI-10] (ab20346) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : VIM (Vimentin) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa

    Lanes 1 - 2: Merged signal (red and green). Green - ab20346 observed at 57 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab20346 was shown to specifically react with Vimentin in wild-type HAP1 cells as signal was lost in VIM (Vimentin) knockout cells. Wild-type and VIM (Vimentin) knockout samples were subjected to SDS-PAGE. Ab20346 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ab20346 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab20346 at 1μg/ml dilution and ab202272 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • ab20346 at a 1/500 dilution staining mouse dissociated neural precursor cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor 568 (red stain) ab175473>ab175473). The image also shows a blue nuclear counterstain. Confocal image is shown with 2x zoom detail in top right corner.

    This image is courtesy of an Abreview submitted by Randal Moldich on 22 February 2006.

    See Abreview

  • Human normal skin. Staining is localised to cytoplasm. Left panel: with primary antibody at 1 ug/mL. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab20346 used at a 1/1000 dilution in Western Blot.5-20µg of total protein from each sample was loaded.

  • Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunofluorescence staining of RBL rat basophiilioc cell line with anti-Vimentin (VI--10). Nuclei are stained with DAPI (blue).



This product has been referenced in:
  • Gan L  et al. Long Non-Coding RNA ZEB1-Antisense 1 Affects Cell Migration and Invasion of Cervical Cancer by Regulating Epithelial-Mesenchymal Transition via the p38MAPK Signaling Pathway. Gynecol Obstet Invest 84:136-144 (2019). Read more (PubMed: 30253398) »
  • Chen Y  et al. miR-300 regulates tumor proliferation and metastasis by targeting lymphoid enhancer-binding factor 1 in hepatocellular carcinoma. Int J Oncol 54:1282-1294 (2019). Read more (PubMed: 30968150) »
See all 51 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A


The protocol we have for GMA staining is as follows;

Place biopsy immediately in ice cold acetone containing protease inhibitors
Fix overnight -20°C
Replace fixative with acetone (room temperature) 15 min
Place biopsy in Methyl benzoate for 15 minutes (This helps infiltration of GMA into the tissue)
Prepare GMA solution A; 5% methyl benzoate in Glycol methacrylate at 4C.
Prepare embedding solution
GMA solution A (10ml) + benzoyl peroxide 70mg
Dossolve the benzoyl peroxde in solution A by gentle shaking
Embed speciomens in freshly prepared embedding solution in Taab flat bottomed capsules, placing biopsy in the bottom of the capsule and filling to the brim with resin and closing lid to exclude air.
Polymerise at 4C for 48 hours.
Store in airtight boxes at -20C.

Section cutting

Remove block from embedding capsules
Trim away the excess resin
Cut 2um sections and float out onto ammonia water (1ml ammonia in 500ml distilled water) for 1-1.5 mins.
Pick sections up onto labelled poly-L-lysine coated slides.
Dry for at ;east 1 hour at RT
Stainin imidiately


Inhibit endogenous perxodase using 0.3% H2O2 for 30 mins.
Wash slides with TBS 3x5 min
Apply primary antibody overnight
Wash slides with TBS 3x5 mins
Apply biotinylated secondary antibody or conjugated antibody as required
Wash with TBS 3x5 mins
Use DAP or AEC substares as required
Counterstain the slide with Hematixylin and mount in DPX.
Store in airtight boxes at -20C.

The following anti Fibroblast antibodies we have in catalogue

ab44971, ab11333, ab8069, ab20346, ab7752, ab92547 etc.

These antibodies unfortunatley aren't tested with GMA staining.

Read More


The lysis was done by 1% lauryl maltoside + protease inhibitors. I am sorry we do not have any further information regarding the amount of the cells in the lysate. For immunoprecipitation 200 microliters of the lysate was incubated with 2 micrograms of VI-10 for 2-4 hours in cold room while mixing, and than 50 microliters of anti-mouse IgM agarose was used. Washing was done using PBS + 0.05% Tween 20, elution was done by 0.1 M glycine, 0.15 M NaCl, pH 2.4.

Read More


From the reference listed on our datasheet, Draberova E et al. Monoclonal antibody VI-10 specific for vimentin. Folia Biol (Praha) 45:35-6 (1999). PubMed: 10732717 we have the following information: "BALB/c mice were immunized with a synthetic peptide corresponding to human gamma-tubulin sequence 38-53, coupled to carrier protein and fused with Sp2/0 myeloma cells... Immunoblotting analysis showed that antibody VI-10 reacted with purified vimentin from Elulich ascitic tumour cells as well as with 57 kDa proteins at the position of vimentin in whole cell lysates."

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up