Western blot abreview for Anti-Vimentin antibody [VI-10]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast)
Loading amount
10 µg
Specification
Breast
Gel Running Conditions
Non-reduced Denaturing (4-8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-mouse 2nd ab
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 54 kDa

Mr. Yoon Pyo Choi

Verified customer

Submitted Jun 25 2009

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