Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neural Stem Cells)
Specification
Neural Stem Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-TritonX 0.2% 10 min
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS-tritonX 0.2% Goat serum 1%

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti mouse IgG1
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
counterstained with Hoechst to identify nuclei

Mrs. Alison Stacey

Verified customer

Submitted Jan 07 2010

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