Western blot abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse cardiac fibroblasts)
Loading amount
20 µg
Specification
mouse cardiac fibroblasts
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 55 kDa

Additional data

Additional Notes
Very good antibody for WB. Gives a very specific band at the correct size with no unspecific bands.

Each lane contains cardiac fibroblast cell lysate. Intensity of the two first lane is lower although same protein amount was loaded on the gel. This might be due to the fact that the blot was striped several times before applying the vimentin Ab.

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Submitted Apr 02 2012

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