Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [VI-10]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Human Cell (Colon carcinoma)
Specification
Colon carcinoma
Permeabilization
Yes - 0.5% Triton in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% BSA in PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Mouse FITC
Host species: Mouse
Clonality: Monoclonal
Conjugation: Fluorescein
Dilution
1/100

Additional data

Abcam response
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Dr. Eleni Petsalaki

Verified customer

Submitted Dec 04 2012

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