Flow Cytometry abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Application
Flow Cytometry
Fixation
Frozen no other fixative
Permeabilization
Yes - 0.1% Triton-X
Sample
Human Cell (Brain)
Specification
Brain
Gating Strategy
forward scatter/side scatter (all cells)
Preparation
Cell harvesting/tissue preparation method: Homogenized frozen tissue
Sample buffer: Tris/EDTA/sucrose/Triton

Other product details

Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS with BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Abcam user community

Verified customer

Submitted Sep 06 2013

Sign up