Western blot abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12,5)
Sample
Human Tissue lysate - whole (human Urether)
Specification
human Urether
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + Tween
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti Mouse, Sigma A2554
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000

Detection

Detection method
ECL
Bands
Specific: 54 kDa
Exposure
3 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Nov 11 2013

Sign up