PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
Key features and details
- PE Mouse monoclonal [VI-RE/1] to Vimentin
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-Vimentin antibody [VI-RE/1]
See all Vimentin primary antibodies -
Description
PE Mouse monoclonal [VI-RE/1] to Vimentin -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types. -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Pig -
Immunogen
Recombinant full length protein corresponding to Human Vimentin.
Database link: P08670 -
Positive control
- ICC/IF: HeLa cells Flow cyt: LEP-19 cells.
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General notes
Conjugated with R-Phycoerythrin (PE) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purified antibodies – protein A (G) affinity chromatography before conjugation. Conjugated antibodies –site exclusion chromatography. -
Clonality
Monoclonal -
Clone number
VI-RE/1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
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|
Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Omim: 193060 Human
- SwissProt: P08670 Human
- Unigene: 455493 Human
- Unigene: 691131 Human
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Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Flow cytometry analysis (intracellular staining) of Vimentin in LEP-19 human fibroblast cell line using ab49918 anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab49918 has been referenced in 5 publications.
- Guo YX et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
- Cervelló I et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . PubMed: 21712999
- Basu D et al. Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas. Cell Cycle 10:2008-16 (2011). PubMed: 21558812
- Basu D et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . PubMed: 20498638
- Meyer MJ et al. Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines. Breast Cancer Res 11:R82 (2009). PubMed: 19906290