Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-Vimentin (phospho S38) antibody [EP1069Y]
See all Vimentin primary antibodies -
DescriptionRabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
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Host speciesRabbit
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Specificityab52942 only detects Vimentin phosphorylated at Serine 38.
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Tested applicationsSuitable for: WB, Dot blot, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
Database link: P08670 -
Positive control
- WB: HeLa cell lysate treated with Calyculin A.
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General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1069Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-Vimentin (phospho S38) antibody [EP1069Y] (Alexa Fluor® 488) (ab205737)
- Anti-Vimentin (phospho S38) antibody [EP1069Y] (Alexa Fluor® 647) (ab205738)
- Anti-Vimentin (phospho S38) antibody [EP1069Y] (Alexa Fluor® 594) (ab207029)
- Anti-Vimentin (phospho S38) antibody [EP1069Y] (Alexa Fluor® 555) (ab208498)
- Anti-Vimentin (phospho S38) antibody [EP1069Y] (Alexa Fluor® 568) (ab208499)
- Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab52942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). | |
| Dot blot | 1/1000. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 24073199 |
Target
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FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
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Involvement in diseaseCataract 30
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Sequence similaritiesBelongs to the intermediate filament family.
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DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
see all -
FormVimentin is found in connective tissue and in the cytoskeleton.
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Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-284752-ab52942WBv2.jpg)
Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution
Lane 1 : HeLa whole cell lysate - untreated
Lane 2 : HeLa whole cell lysate - treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-298800-ab5294260000.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)This image is courtesy of an Abreview submitted by Kirk Mcmanusab52942 staining Vimentin (phospho S38) in the hTERT fibroblastsl cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/200). ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal was used as the secondary antibody (1/200). Cells were counterstained with DAPI.
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![Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-255472-ab52942Dot.jpg)
Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942.gif)
Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate Calyculin A treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Datasheets and documents
References
This product has been referenced in:
- Chi J et al. Classical intracranial chondrosarcoma: A case report. Oncol Lett 12:4051-4053 (2016). IHC ; Human . Read more (PubMed: 27895770) »
- Kajita M et al. Filamin acts as a key regulator in epithelial defence against transformed cells. Nat Commun 5:4428 (2014). Read more (PubMed: 25079702) »
