Recombinant
RabMAb

Recombinant Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Reviews (1) Submit a question References (5)

Overview

  • Product name

    Anti-Vimentin (phospho S38) antibody [EP1069Y]
    See all Vimentin primary antibodies

  • Description

    Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)

  • Host species

    Rabbit

  • Specificity

    ab52942 only detects Vimentin phosphorylated at Serine 38.

  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
    Database link: P08670

  • Positive control

    • WB: HeLa cell lysate treated with Calyculin A.

  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Dot blot 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.

    • Target

    • Function

      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

    • Tissue specificity

      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

    • Involvement in disease

      Cataract 30

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Domain

      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

    • Post-translational
      modifications

      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Form

      Vimentin is found in connective tissue and in the cytoskeleton.

    • Alternative names

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Images

    • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
      Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
      All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

      Lane 1 : HeLa whole cell lysate - untreated
      Lane 2 : HeLa whole cell lysate - treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
      Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

      Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
      Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
      All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate Calyculin A treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    References

    This product has been referenced in:

    See all 5 Publications for this product

    Publishing research using ab52942? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin (phospho S38) antibody [EP1069Y]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hTERT fibroblasts)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    hTERT fibroblasts
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Dec 08 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com