Recombinant Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-284752-anti-vimentin-phospho-s38-antibody-ep1069y-western-blot.jpg "Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Vimentin (phospho S38) antibody [EP1069Y]
See all Vimentin primary antibodies -
Description
Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
Database link: P08670 -
Positive control
- WB: HeLa cell lysate treated with Calyculin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1069Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
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| Dot blot |
1/1000.
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| Notes |
|---|
|
WB
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
|
Dot blot
1/1000. |
Application notes
Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
see all -
Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution
Lane 1 : HeLa whole cell lysate - untreated
Lane 2 : HeLa whole cell lysate - treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-255472-anti-vimentin-phospho-s38-antibody-ep1069y-dot-blot.jpg)
Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-65462-anti-vimentin-phospho-s38-antibody-ep1069y-western-blot.gif)
Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate Calyculin A treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
![Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.com/ps/products/52/ab52942/Images/ab52942-6-benefits-of-recombinant-antibodies.png)
Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab52942 has been referenced in 6 publications.
- Liu J et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
- Chi J et al. Classical intracranial chondrosarcoma: A case report. Oncol Lett 12:4051-4053 (2016). IHC ; Human . PubMed: 27895770
- Kajita M et al. Filamin acts as a key regulator in epithelial defence against transformed cells. Nat Commun 5:4428 (2014). PubMed: 25079702
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). ICC/IF ; Human . PubMed: 24073199
- Mähönen AJ et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502
- Kim H et al. Filamin A is required for vimentin-mediated cell adhesion and spreading. Am J Physiol Cell Physiol 298:C221-36 (2010). PubMed: 19776392
