Name: Anti-Vimentin (phospho S38) antibody [EP1069Y]
Brand: RabMAb
SKU: ab52942
Rating: 3 (1 reviews)

Product name

Anti-Vimentin (phospho S38) antibody [EP1069Y]
See all Vimentin primary antibodies
Description

Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
Host species

Rabbit
Specificity

ab52942 only detects Vimentin phosphorylated at Serine 38.
Tested applications

Suitable for: WB, Dot blot, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
Database link: P08670
Positive control
- WB: HeLa cell lysate treated with Calyculin A.
General notes

A trial size is available to purchase for this antibody.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1069Y
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab52942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Dot blot		1/1000.
ICC/IF		Use at an assay dependent concentration. PubMed: 24073199

Application notes

Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Involvement in disease

Cataract 30
Sequence similarities

Belongs to the intermediate filament family.
Domain

The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
Post-translational
modifications

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P08670 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
- Unigene: 691131 Human
- Unigene: 268000 Mouse
- Unigene: 2710 Rat
see all
Form

Vimentin is found in connective tissue and in the cytoskeleton.
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
- HEL113 antibody
- VIM antibody
- VIME_HUMAN antibody
- Vimentin antibody
see all

Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

Lane 1 : HeLa whole cell lysate - untreated
Lane 2 : HeLa whole cell lysate - treated with Calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)This image is courtesy of an Abreview submitted by Kirk Mcmanus

ab52942 staining Vimentin (phospho S38) in the hTERT fibroblastsl cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/200). ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal was used as the secondary antibody (1/200). Cells were counterstained with DAPI.
See Abreview
Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate Calyculin A treated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

Datasheet

This product has been referenced in:

Chi J et al. Classical intracranial chondrosarcoma: A case report. Oncol Lett 12:4051-4053 (2016). IHC ; Human . Read more (PubMed: 27895770) »
Kajita M et al. Filamin acts as a key regulator in epithelial defence against transformed cells. Nat Commun 5:4428 (2014). Read more (PubMed: 25079702) »

See all 5 Publications for this product

Publishing research using ab52942? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (hTERT fibroblasts)

Permeabilization

Yes - 0.5% Triton X-100

Specification

hTERT fibroblasts

Fixative

Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Overview

Properties

Associated products

Applications

Target

Images

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin (phospho S38) antibody [EP1069Y]