RecombinantRabMAb

Recombinant Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

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Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
  • Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
  • Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Vimentin (phospho S38) antibody [EP1069Y]
    See all Vimentin primary antibodies

  • Description

    Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
    Database link: P08670

  • Positive control

    • WB: HeLa cell lysate treated with Calyculin A.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Dot blot
1/1000.
Notes
WB
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Dot blot
1/1000.
Application notes
Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • Involvement in disease

    Cataract 30

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.

  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

    Lane 1 : HeLa whole cell lysate - untreated
    Lane 2 : HeLa whole cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

    Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate Calyculin A treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab52942? Please let us know so that we can cite the reference in this datasheet.

ab52942 has been referenced in 6 publications.

  • Liu J  et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
  • Chi J  et al. Classical intracranial chondrosarcoma: A case report. Oncol Lett 12:4051-4053 (2016). IHC ; Human . PubMed: 27895770
  • Kajita M  et al. Filamin acts as a key regulator in epithelial defence against transformed cells. Nat Commun 5:4428 (2014). PubMed: 25079702
  • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). ICC/IF ; Human . PubMed: 24073199
  • Mähönen AJ  et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502
  • Kim H  et al. Filamin A is required for vimentin-mediated cell adhesion and spreading. Am J Physiol Cell Physiol 298:C221-36 (2010). PubMed: 19776392

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin (phospho S38) antibody [EP1069Y]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT fibroblasts)
Permeabilization
Yes - 0.5% Triton X-100
Specification
hTERT fibroblasts
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Dec 08 2017

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