Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)

Overview

  • Product name

    Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free
    See all Vimentin primary antibodies
  • Description

    Rabbit monoclonal [EP1070Y] to Vimentin (phospho S72) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Vimentin aa 50-150 (phospho P72). The exact sequence is proprietary.

  • Positive control

    • WB: C6 (starved for 24 hours then treated with 100 ng Calyculin A for 60 minutes) whole cell lysate.
  • General notes

    Ab232451 is the carrier-free version of ab52944. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use at an assay dependent concentration.

For unpurified use at 1:40.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/10000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : C6 (starved for 24 hours then treated with 100 ng Calyculin A for 60 minutes) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer : 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

  • ab52944 (purified) at 1:20 dilution (2µg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
    Lane 2 (+): ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

  • Primary antibody dilution: 1/1000
    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
    Blocking & dilutiing buffer: 5% NFDM/TBST

    Lane 1 sample: Vimentin (pS72) phospho peptide
    Lane 2 sample: Vimentin non-phospho peptide

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

References

ab232451 has not yet been referenced specifically in any publications.

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