Key features and details
- Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Vimentin (phospho S82) antibody [EP1071Y]
See all Vimentin primary antibodies
DescriptionRabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
Specificityab52943 only detects Vimentin phosphorylated at Serine 82.
Tested applicationsSuitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Vimentin (phospho S82). The exact sequence is proprietary.
- WB: AP treated and untreated HeLa + Calyculin A cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for Flow Cyt, IHC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Involvement in diseaseCataract 30
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
FormVimentin is found in connective tissue and in the cytoskeleton.
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: Vimentin (pS82) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide
Exposure time: 3 minutes
All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution
Lane 1 : HeLa cell lysate AP treated
Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52943 has been referenced in 3 publications.
- Goto H et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). PubMed: 26795468
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB, ICC/IF ; Human . PubMed: 24073199
- Mähönen AJ et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502