Non-Abcam antibody was used: GAR Alexa 790 conjugated secondary Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor 790
1 minute(s) and 0 second(s)
Specific: ~100 kDa
Ab200812 produced a band around the expected molecular weight (left). However, this band migrated faster (appeared as lower MW) than total vinculin protein detected by a separate antibody (middle, right). As phosphoprotein bands usually migrate slower than the total protein bands, it is inconclusive if AB200812 is detecting PY100-vinculin in pigs.
Thank you for submitting your feedback on use of ab200812 in pig samples. We have not validated this antibody in pig, and will continue to collect data on use in this species.
Abcam user community
Submitted Apr 14 2017
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