Overview

  • Product name

    Anti-Vinculin (phospho Y822) antibody
    See all Vinculin primary antibodies
  • Description

    Rabbit polyclonal to Vinculin (phospho Y822)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Chicken
    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Vinculin (phospho Y822). chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 822.
    Database link: P18206

  • Positive control

    • Chick Embryo Fibroblasts (CEFs) transfected with activated Src.

Properties

Applications

Our Abpromise guarantee covers the use of ab200825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 124 kDa.

Target

  • Function

    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • Tissue specificity

    Metavinculin is muscle-specific.
  • Involvement in disease

    Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities

    Belongs to the vinculin/alpha-catenin family.
  • Domain

    Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
    The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.
  • Post-translational
    modifications

    Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
    Aceylated; mainly by myristic acid but also small amount of palmitic acid.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMD1W antibody
    • CMH15 antibody
    • Epididymis luminal protein 114 antibody
    • HEL114 antibody
    • Metavinculin antibody
    • MV antibody
    • MVCL antibody
    • OTTHUMP00000019861 antibody
    • OTTHUMP00000019862 antibody
    • VCL antibody
    • VINC antibody
    • VINC_HUMAN antibody
    • Vinculin antibody
    see all

Images

  • All lanes : Anti-Vinculin (phospho Y822) antibody (ab200825) at 1/1000 dilution

    Lane 1 : Untransfected CEF lysates
    Lane 2 : Src transfected CEF lysates
    Lane 3 : Src transfected CEF lysates with non-phosphopeptide corresponding to immunogen
    Lane 4 : Src transfected CEF lysates with generic phosphotyrosine containing peptide
    Lane 5 : Src transfected CEF lysates with phosphopeptide immunogen

    Secondary
    Lanes 1-2 : goat F(ab’)2 anti-rabbit IgG HRP conjugate


    Developed using the ECL technique.

    Predicted band size: 124 kDa



    10% polyacrylamide gel transferred to PVDF blocked with a 5% BSA-TBST

References

ab200825 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human pulmonary endothelial cells)
Gel Running Conditions
Non-reduced Denaturing (7.5% gel)
Loading amount
250000 cells
Treatment
Force perturbation for 30min
Specification
Human pulmonary endothelial cells
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 09 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2016

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