Overview

  • Product name

  • Description

    Rabbit polyclonal to Vinexin
  • Host species

    Rabbit
  • Specificity

    This antibody should recognize isoform Beta (O60504-2) and isoform alpha (O60504-1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Vinexin (internal sequence).
    Database link: O60504

  • Positive control

    • NT2D1 and IMR32 whole cell lysates; Human Colon carcinoma tissue.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM

Target

  • Relevance

    Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
  • Cellular localization

    Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
  • Database links

  • Alternative names

    • SCAM 1 antibody
    • SCAM1 antibody
    • SH3 containing adapter molecule 1 antibody
    • SH3 domain containing adapter molecule 1 antibody
    • SH3D4 antibody
    • Sorbin and SH3 domain containing 3 antibody
    • Sorbin and SH3 domain containing protein 3 antibody
    • Sorbs3 antibody
    • Vinexin alpha antibody
    • Vinexin antibody
    • Vinexin beta (SH3 containing adaptor molecule 1) antibody
    see all

Images

  • Anti-Vinexin antibody (ab126971) at 1/2000 dilution + IMR32 whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 75 kDa



    10% SDS PAGE
  • ab126971, at 1/250 dilution, staining Vinexin in paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry.

References

ab126971 has not yet been referenced specifically in any publications.

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