Key features and details
- Rabbit polyclonal to Vinexin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Vinexin antibody
See all Vinexin primary antibodies
DescriptionRabbit polyclonal to Vinexin
SpecificityThis antibody should recognize isoform Beta (O60504-2) and isoform alpha (O60504-1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Vinexin (internal sequence).
Database link: O60504
- NT2D1 and IMR32 whole cell lysates; Human Colon carcinoma tissue.
General notesKeep as concentrated solution.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 75 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM|
RelevanceVinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
Cellular localizationLocalized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
- SCAM 1 antibody
- SCAM1 antibody
- SH3 containing adapter molecule 1 antibody
ab126971 has not yet been referenced specifically in any publications.