Overview

  • Product name

  • Description

    Rabbit polyclonal to Vinexin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 58-263 of Human Vinexin (O60504).

  • Positive control

    • HepG2 whole cell lysate; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
  • Cellular localization

    Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
  • Database links

  • Alternative names

    • SCAM 1 antibody
    • SCAM1 antibody
    • SH3 containing adapter molecule 1 antibody
    • SH3 domain containing adapter molecule 1 antibody
    • SH3D4 antibody
    • Sorbin and SH3 domain containing 3 antibody
    • Sorbin and SH3 domain containing protein 3 antibody
    • Sorbs3 antibody
    • Vinexin alpha antibody
    • Vinexin antibody
    • Vinexin beta (SH3 containing adaptor molecule 1) antibody
    see all

Images

  • Anti-Vinexin antibody (ab126986) at 1/500 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 75 kDa



    10% SDS PAGE
  • ab126986 at a 1/250 dilution staining Vinexin in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

ab126986 has not yet been referenced specifically in any publications.

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