Product nameAnti-Vinexin antibody
See all Vinexin primary antibodies
DescriptionMouse polyclonal to Vinexin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Vinexin protein (Human)
- Vinexin transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 75 kDa).|
RelevanceVinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
Cellular localizationLocalized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
- SCAM 1 antibody
- SCAM1 antibody
- SH3 containing adapter molecule 1 antibody
All lanes : Anti-Vinexin antibody (ab68222) at 1/500 dilution
Lane 1 : Vinexin transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 75 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab68222 has not yet been referenced specifically in any publications.