violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)
![Flow Cytometry - violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)](https://www.abcam.com/ps/products/241/ab241939/Images/ab241939-330776-anti-cd451-antibody-a20-violetfluor-450-flow-cytometry.jpg "Flow Cytometry - violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)")
Key features and details
- violetFluor™ 450 Mouse monoclonal [A20] to CD45.1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG2a
Overview
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Product name
violetFluor™ 450 Anti-CD45.1 antibody [A20]
See all CD45.1 primary antibodies -
Description
violetFluor™ 450 Mouse monoclonal [A20] to CD45.1 -
Host species
Mouse -
Conjugation
violetFluor™ 450 -
Specificity
ab241939 has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: SJL splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
A20 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD45.1 antibody [A20] (ab210237)
- FITC Anti-CD45.1 antibody [A20] (ab24917)
- Cy5 ® Anti-CD45.1 antibody [A20] (ab24982)
- Biotin Anti-CD45.1 antibody [A20] (ab25011)
- Anti-CD45.1 antibody [A20] (ab25078)
- APC Anti-CD45.1 antibody [A20] (ab25348)
- PE/Cy5® Anti-CD45.1 antibody [A20] (ab25349)
- APC/Cy5.5® Anti-CD45.1 antibody [A20] (ab81989)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab241939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use µl for 10 cells. Use 0.5 µg/test. |
Target
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Relevance
CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
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Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)
Flow cytometric analysis of SJL splenocytes labeling CD45.1 with 0.5 µg ab241939 (solid line) or 0.5 µg violetFluor™ 450 Mouse IgG2a isotype control (dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241939 has not yet been referenced specifically in any publications.
