violetFluor™ 450 Anti-CD8 alpha antibody [2.43] (ab242255)
![Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [2.43] (ab242255)](https://www.abcam.com/ps/products/242/ab242255/Images/ab242255-331239-anti-cd8-alpha-antibody-243-violetfluor-450-flow-cytometry.jpg "Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [2.43] (ab242255)")
Key features and details
- violetFluor™ 450 Rat monoclonal [2.43] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG2b
Overview
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Product name
violetFluor™ 450 Anti-CD8 alpha antibody [2.43]
See all CD8 alpha primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [2.43] to CD8 alpha -
Host species
Rat -
Conjugation
violetFluor™ 450 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2.43 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 0.25 μg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [2.43] (ab242255)
Flow Cytometric analysis of C57Bl/6 bone marrow cells staining CD8 using 0.25 µg ab242255 (solid line) or 0.25 µg violetFluor™ 450 Rat IgG2b isotype control (dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab242255 has been referenced in 1 publication.
- Xu X et al. Tumor Cells Modified with Newcastle Disease Virus Expressing IL-24 as a Cancer Vaccine. Mol Ther Oncolytics 14:213-221 (2019). PubMed: 31338417
