violetFluor™ 450 Anti-CD8 alpha antibody [OKT8] (ab241101)
![Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [OKT8] (ab241101)](https://www.abcam.com/ps/products/241/ab241101/Images/ab241101-322799-anti-cd8-alpha-antibody-okt8-violetfluor-450-flow-cytometry.jpg "Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [OKT8] (ab241101)")
Key features and details
- violetFluor™ 450 Mouse monoclonal [OKT8] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: violetFluor™ 450
- Isotype: IgG2a
Overview
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Product name
violetFluor™ 450 Anti-CD8 alpha antibody [OKT8]
See all CD8 alpha primary antibodies -
Description
violetFluor™ 450 Mouse monoclonal [OKT8] to CD8 alpha -
Host species
Mouse -
Conjugation
violetFluor™ 450 -
Specificity
ab241101 is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
If used together with alternative antibodies Anti-Human CD8 alpha clone RPA-T8 or Anti-Human CD8 alpha clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
OKT8 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Use 5 µl (0.125 µg)/test. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-CD8 alpha antibody [OKT8] (ab241101)
Flow cytometric analysis of human peripheral blood lymphocytes stained for CD8 alpha with 5 µl (0.125 µg) ab241101 (solid line) or 0.125 µg violetFluor™ 450 Mouse IgG2a isotype control (dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241101 has not yet been referenced specifically in any publications.
