violetFluor™ 450 Anti-F4/80 antibody [BM8.1] (ab242258)
![Flow Cytometry - violetFluor™ 450 Anti-F4/80 antibody [BM8.1] (ab242258)](https://www.abcam.com/ps/products/242/ab242258/Images/ab242258-331251-anti-f4-80-antibody-bm81-violetfluor-450-flow-cytometry.jpg "Flow Cytometry - violetFluor™ 450 Anti-F4/80 antibody [BM8.1] (ab242258)")
Key features and details
- violetFluor™ 450 Rat monoclonal [BM8.1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
- Isotype: IgG2b
Overview
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Product name
violetFluor™ 450 Anti-F4/80 antibody [BM8.1]
See all F4/80 primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [BM8.1] to F4/80 -
Host species
Rat -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 bone marrow cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BM8.1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-F4/80 antibody [BM8.1] (ab242258)
Flow Cytometric analysis of C57Bl/6 bone marrow cells staining F4/80 using PerCP-Cy5.5 Anti-Mouse CD11b and 0.5 µg ab242258 (right panel) or 0.5 µg violet- Fluor™ 450 Rat IgG2b isotype control (left panel).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab242258 has not yet been referenced specifically in any publications.